Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts.


Journal

BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232

Informations de publication

Date de publication:
12 Feb 2021
Historique:
received: 09 05 2020
accepted: 02 02 2021
entrez: 13 2 2021
pubmed: 14 2 2021
medline: 8 9 2021
Statut: epublish

Résumé

Euphorbia helioscopia, conventionally known as sun spurge, has been used as a traditional medicine to treat different diseases owing to its reported antitumor, antiviral and antioxidant activities. The current research was formulated to assess the in-vitro antioxidant and antidiabetic ability of Euphorbia helioscopia subsequent to the phytochemical analysis of its various extracts. For this purpose, methanol, ethanol and aqueous extracts were prepared using the whole dried plant. Phytochemical analysis of the extracts was done to evaluate the total flavonoid components (TFC) and total phenolic components (TPC) in the extracts. A total of seven phenolic and three flavonoid contents were documented and quantified using HPLC. Antioxidant values were found by DPPH After analyzing statistically, the results showed that methanolic extract possesses the highest TFC and TPC values while aqueous extract encompassed the lowest level of these contents. Invitro results showed that methanolic extract of the Euphorbia helioscopia has the maximum antioxidant capability since it showed the highest scavenging ability towards the DPPH The experiment outcomes have shown that Euphorbia helioscopia extracts used in the current study contain antioxidant and antidiabetic activities; however, it is highest in its methanolic extract. The presence of the same trend towards the highest antidiabetic activity of the methanolic extract in terms of maximum inhibiting activity of α amylase and α glucosidase enzymes suggests a close association of TFC and TPC in minimizing diabetes.

Sections du résumé

BACKGROUND BACKGROUND
Euphorbia helioscopia, conventionally known as sun spurge, has been used as a traditional medicine to treat different diseases owing to its reported antitumor, antiviral and antioxidant activities.
METHODS METHODS
The current research was formulated to assess the in-vitro antioxidant and antidiabetic ability of Euphorbia helioscopia subsequent to the phytochemical analysis of its various extracts. For this purpose, methanol, ethanol and aqueous extracts were prepared using the whole dried plant. Phytochemical analysis of the extracts was done to evaluate the total flavonoid components (TFC) and total phenolic components (TPC) in the extracts. A total of seven phenolic and three flavonoid contents were documented and quantified using HPLC. Antioxidant values were found by DPPH
RESULTS RESULTS
After analyzing statistically, the results showed that methanolic extract possesses the highest TFC and TPC values while aqueous extract encompassed the lowest level of these contents. Invitro results showed that methanolic extract of the Euphorbia helioscopia has the maximum antioxidant capability since it showed the highest scavenging ability towards the DPPH
CONCLUSION CONCLUSIONS
The experiment outcomes have shown that Euphorbia helioscopia extracts used in the current study contain antioxidant and antidiabetic activities; however, it is highest in its methanolic extract. The presence of the same trend towards the highest antidiabetic activity of the methanolic extract in terms of maximum inhibiting activity of α amylase and α glucosidase enzymes suggests a close association of TFC and TPC in minimizing diabetes.

Identifiants

pubmed: 33579270
doi: 10.1186/s12906-021-03237-x
pii: 10.1186/s12906-021-03237-x
pmc: PMC7881459
doi:

Substances chimiques

Antioxidants 0
Biphenyl Compounds 0
Flavonoids 0
Hypoglycemic Agents 0
Phenols 0
Phytochemicals 0
Picrates 0
Plant Extracts 0
1,1-diphenyl-2-picrylhydrazyl DFD3H4VGDH
alpha-Amylases EC 3.2.1.1
alpha-Glucosidases EC 3.2.1.20
Methanol Y4S76JWI15

Types de publication

Comparative Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

62

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Auteurs

Imtiaz Mustafa (I)

Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.

Muhammad Naeem Faisal (MN)

Institute of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan.

Ghulam Hussain (G)

Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.

Humaira Muzaffar (H)

Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.

Muhammad Imran (M)

Department of Food Science, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.

Muhammad Umar Ijaz (MU)

Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan.

Muhammad Umar Sohail (MU)

Biomedical Research Centre, Qatar University, Doha, Qatar.

Arslan Iftikhar (A)

Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.

Arslan Shaukat (A)

Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.

Haseeb Anwar (H)

Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan. drhaseebanwar@gcuf.edu.pk.

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