Flavoprotein monooxygenases: Versatile biocatalysts.
(hydro)peroxide
Baeyer-Villiger oxidation
biocatalysis
dearomatization
epoxidation
flavin
halogenation
hydroxylation
microbial degradation
oxygenation
Journal
Biotechnology advances
ISSN: 1873-1899
Titre abrégé: Biotechnol Adv
Pays: England
ID NLM: 8403708
Informations de publication
Date de publication:
01 11 2021
01 11 2021
Historique:
received:
13
11
2020
revised:
27
01
2021
accepted:
06
02
2021
pubmed:
16
2
2021
medline:
3
11
2021
entrez:
15
2
2021
Statut:
ppublish
Résumé
Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set of chemo-, regio- and enantioselective oxyfunctionalization reactions. In this review, we describe how FPMOs have evolved from model enzymes in mechanistic flavoprotein research to biotechnologically relevant catalysts that can be applied for the sustainable production of valuable chemicals. After a historical account of the development of the FPMO field, we explain the FPMO classification system, which is primarily based on protein structural properties and electron donor specificities. We then summarize the most appealing reactions catalyzed by each group with a focus on the different types of oxygenation chemistries. Wherever relevant, we report engineering strategies that have been used to improve the robustness and applicability of FPMOs.
Identifiants
pubmed: 33588053
pii: S0734-9750(21)00018-5
doi: 10.1016/j.biotechadv.2021.107712
pii:
doi:
Substances chimiques
Flavoproteins
0
Mixed Function Oxygenases
EC 1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
107712Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.