Meiotic arrest with roscovitine and sexual maturity improve competence of mouse oocytes by regulating expression of competence-related genes.
Animals
Blastocyst
Cell Nucleolus
/ metabolism
Chromatin
/ metabolism
Coculture Techniques
Cumulus Cells
/ metabolism
Female
Gene Expression Profiling
Gene Expression Regulation
In Vitro Oocyte Maturation Techniques
/ methods
Meiosis
/ drug effects
Mice
Oocytes
/ cytology
Ovarian Follicle
/ metabolism
Roscovitine
/ pharmacology
Transcription, Genetic
Chromatin configuration
Gene expression
Meiotic arrest
Oocyte competence
Roscovitine
Journal
The Journal of reproduction and development
ISSN: 1348-4400
Titre abrégé: J Reprod Dev
Pays: Japan
ID NLM: 9438792
Informations de publication
Date de publication:
21 Apr 2021
21 Apr 2021
Historique:
pubmed:
19
2
2021
medline:
21
12
2021
entrez:
18
2
2021
Statut:
ppublish
Résumé
We have studied the mechanisms by which meiotic arrest maintenance (MAM) with roscovitine, female sexual maturity, and the surrounded nucleoli (SN) chromatin configuration improve the competence of mouse oocytes by observing the expression of oocyte competence-related genes in non-surrounded nucleoli (NSN) and SN oocytes from prepubertal and adult mice following maturation with or without MAM. The results demonstrated that MAM with roscovitine significantly improved the developmental potential of adult SN and prepubertal NSN oocytes, but had no effect on that of prepubertal SN oocytes. Without MAM, while 40% of the 2-cell embryos derived from prepubertal SN oocytes developed into 4-cell embryos, none of the 2-cell embryos derived from prepubertal NSN oocytes did, and while 42% of the 4-cell embryos derived from adult SN oocytes developed into blastocysts, only 1% of the 4-cell embryos derived from prepubertal SN oocytes developed into blastocysts. Furthermore, MAM with roscovitine, SN configuration, and female sexual maturity significantly increased the mRNA levels of competence-beneficial genes and decreased those of competence-detrimental genes. In conclusion, our results suggest that MAM with roscovitine, SN chromatin configuration, and female sexual maturity improve oocyte competence by regulating the expression of competence-related genes, suggesting that Oct4, Stella, Mater, Zar1, Mapk8, and Bcl2 are oocyte competence-beneficial genes, whereas Foxj2, Ship1, and Bax are competence-detrimental genes.
Identifiants
pubmed: 33597332
doi: 10.1262/jrd.2020-142
pmc: PMC8075721
doi:
Substances chimiques
Chromatin
0
Roscovitine
0ES1C2KQ94
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115-122Références
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