Transcriptomic response of Saccharomyces cerevisiae to octanoic acid production.
RNA-Seq
RPL40B
octanoic acid
transcriptome response to medium-chain fatty acids
Journal
FEMS yeast research
ISSN: 1567-1364
Titre abrégé: FEMS Yeast Res
Pays: England
ID NLM: 101085384
Informations de publication
Date de publication:
18 03 2021
18 03 2021
Historique:
received:
26
11
2020
accepted:
16
02
2021
pubmed:
19
2
2021
medline:
6
11
2021
entrez:
18
2
2021
Statut:
ppublish
Résumé
The medium-chain fatty acid octanoic acid is an important platform compound widely used in industry. The microbial production from sugars in Saccharomyces cerevisiae is a promising alternative to current non-sustainable production methods, however, titers need to be further increased. To achieve this, it is essential to have in-depth knowledge about the cell physiology during octanoic acid production. To this end, we collected the first RNA-Seq data of an octanoic acid producer strain at three time points during fermentation. The strain produced higher levels of octanoic acid and increased levels of fatty acids of other chain lengths (C6-C18) but showed decreased growth compared to the reference. Furthermore, we show that the here analyzed transcriptomic response to internally produced octanoic acid is notably distinct from a wild type's response to externally supplied octanoic acid as reported in previous publications. By comparing the transcriptomic response of different sampling times, we identified several genes that we subsequently overexpressed and knocked out, respectively. Hereby we identified RPL40B, to date unknown to play a role in fatty acid biosynthesis or medium-chain fatty acid tolerance. Overexpression of RPL40B led to an increase in octanoic acid titers by 40%.
Identifiants
pubmed: 33599754
pii: 6144596
doi: 10.1093/femsyr/foab011
pmc: PMC7972946
pii:
doi:
Substances chimiques
Caprylates
0
Saccharomyces cerevisiae Proteins
0
octanoic acid
OBL58JN025
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.
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