Conformation of the von Willebrand factor/factor VIII complex in quasi-static flow.
analytical ultracentrifugation
dynamic light scattering
factor VIII
protein conformation
size-exclusion chromatography
structural model
von Willebrand factor
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
Historique:
received:
31
12
2020
revised:
01
02
2021
accepted:
11
02
2021
pubmed:
19
2
2021
medline:
28
8
2021
entrez:
18
2
2021
Statut:
ppublish
Résumé
Von Willebrand factor (VWF) is a plasma glycoprotein that circulates noncovalently bound to blood coagulation factor VIII (fVIII). VWF is a population of multimers composed of a variable number of ∼280 kDa monomers that is activated in shear flow to bind collagen and platelet glycoprotein Ibα. Electron microscopy, atomic force microscopy, small-angle neutron scattering, and theoretical studies have produced a model in which the conformation of VWF under static conditions is a compact, globular "ball-of-yarn," implying strong, attractive forces between monomers. We performed sedimentation velocity (SV) analytical ultracentrifugation measurements on unfractionated VWF/fVIII complexes. There was a 20% per mg/ml decrease in the weight-average sedimentation coefficient, s
Identifiants
pubmed: 33600794
pii: S0021-9258(21)00193-9
doi: 10.1016/j.jbc.2021.100420
pmc: PMC8005835
pii:
doi:
Substances chimiques
Drug Combinations
0
factor VIII, von Willebrand factor drug combination
0
von Willebrand Factor
0
Factor VIII
9001-27-8
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
100420Subventions
Organisme : NHLBI NIH HHS
ID : U54 HL141981
Pays : United States
Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
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