Transcription activation by a sliding clamp.
Amino Acid Motifs
Base Sequence
DNA Polymerase III
/ chemistry
DNA, Single-Stranded
/ metabolism
DNA, Viral
/ metabolism
DNA-Directed RNA Polymerases
/ metabolism
Escherichia coli
/ enzymology
Models, Genetic
Models, Molecular
Promoter Regions, Genetic
Protein Binding
Protein Domains
Sigma Factor
/ chemistry
Transcription, Genetic
Transcriptional Activation
/ genetics
Viral Proteins
/ chemistry
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 02 2021
18 02 2021
Historique:
received:
15
10
2020
accepted:
26
01
2021
entrez:
19
2
2021
pubmed:
20
2
2021
medline:
7
4
2021
Statut:
epublish
Résumé
Transcription activation of bacteriophage T4 late genes is accomplished by a transcription activation complex containing RNA polymerase (RNAP), the promoter specificity factor gp55, the coactivator gp33, and a universal component of cellular DNA replication, the sliding clamp gp45. Although genetic and biochemical studies have elucidated many aspects of T4 late gene transcription, no precise structure of the transcription machinery in the process is available. Here, we report the cryo-EM structures of a gp55-dependent RNAP-promoter open complex and an intact gp45-dependent transcription activation complex. The structures reveal the interactions between gp55 and the promoter DNA that mediate the recognition of T4 late promoters. In addition to the σR2 homology domain, gp55 has a helix-loop-helix motif that chaperons the template-strand single-stranded DNA of the transcription bubble. Gp33 contacts both RNAP and the upstream double-stranded DNA. Gp45 encircles the DNA and tethers RNAP to it, supporting the idea that gp45 switches the promoter search from three-dimensional diffusion mode to one-dimensional scanning mode.
Identifiants
pubmed: 33602900
doi: 10.1038/s41467-021-21392-0
pii: 10.1038/s41467-021-21392-0
pmc: PMC7892883
doi:
Substances chimiques
DNA, Single-Stranded
0
DNA, Viral
0
Sigma Factor
0
Viral Proteins
0
gene 33 protein, Enterobacteria phage T4
0
gene 55 protein, Enterobacteria phage T4
0
beta subunit, DNA polymerase III
EC 2.7.7.-
DNA-Directed RNA Polymerases
EC 2.7.7.6
DNA Polymerase III
EC 2.7.7.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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