Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers.
Journal
Nature materials
ISSN: 1476-4660
Titre abrégé: Nat Mater
Pays: England
ID NLM: 101155473
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
received:
05
03
2020
accepted:
23
12
2020
pubmed:
20
2
2021
medline:
30
9
2021
entrez:
19
2
2021
Statut:
ppublish
Résumé
Actomyosin machinery endows cells with contractility at a single-cell level. However, within a monolayer, cells can be contractile or extensile based on the direction of pushing or pulling forces exerted by their neighbours or on the substrate. It has been shown that a monolayer of fibroblasts behaves as a contractile system while epithelial or neural progentior monolayers behave as an extensile system. Through a combination of cell culture experiments and in silico modelling, we reveal the mechanism behind this switch in extensile to contractile as the weakening of intercellular contacts. This switch promotes the build-up of tension at the cell-substrate interface through an increase in actin stress fibres and traction forces. This is accompanied by mechanotransductive changes in vinculin and YAP activation. We further show that contractile and extensile differences in cell activity sort cells in mixtures, uncovering a generic mechanism for pattern formation during cell competition, and morphogenesis.
Identifiants
pubmed: 33603188
doi: 10.1038/s41563-021-00919-2
pii: 10.1038/s41563-021-00919-2
pmc: PMC7611436
mid: EMS118803
doi:
Substances chimiques
Actomyosin
9013-26-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1156-1166Subventions
Organisme : European Research Council
ID : 617233
Pays : International
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature Limited.
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