Investigation of genetic variation: Neuraminidase gene of influenza A virus H1N1/pdm09, Shiraz, Iran (2015-2016).
Adolescent
Amino Acid Substitution
Child
Child, Preschool
Female
Genetic Variation
Genotype
Humans
Infant
Influenza A Virus, H1N1 Subtype
/ enzymology
Influenza, Human
/ epidemiology
Iran
/ epidemiology
Male
Mutation
Neuraminidase
/ classification
Phylogeny
RNA, Viral
/ genetics
Sequence Analysis, DNA
Viral Proteins
/ classification
Young Adult
influenza A virus
H1N1
mutation
oseltamivir-resistant
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
revised:
15
02
2021
received:
29
05
2019
accepted:
16
02
2021
pubmed:
20
2
2021
medline:
15
12
2021
entrez:
19
2
2021
Statut:
ppublish
Résumé
Oseltamivir and antiviral agents are frequently used for the prevention and treatment of influenza infection. However, resistance to oseltamivir has been reported globally due to a mutation in the Influenza virus neuraminidase gene. Such resistance will be detected by genotyping and phenotyping studies of viral isolates. The recent study aimed to determine the genetic mutation of neuraminidase gene in influenza A (H1N1) viruses isolated from children referred to Shiraz tertiary hospitals during 1 year (2015-2016) with influenza-like symptoms. A total of 300 patients were registered and throat samples were taken. The throat swabs were used for viral RNA extraction. Detection of influenza A (H1N1) was performed using the one-step real-time polymerase chain reaction (qRT-PCR) method. From positive isolates for H1N1, 51 random samples were evaluated for neuraminidase gene mutation with the nested PCR-sequencing method. Of 300 cases, 102 (34%) isolates were detected as influenza A (H1N1) pdm09. Based on sequencing results, 2 of the 44 sequenced isolates exhibited H275Y substitution, which presented oseltamivir resistance. In comparison with reference strain, the phylogenetic analysis of sequenced isolates was classified in genogroup 6B. While this result is the first report of emerging oseltamivir-resistant in the southwest of Iran, it is highly recommended to perform these evaluations on the different geographical regions in any prevalence area to plan treatment strategies for influenza.
Substances chimiques
RNA, Viral
0
Viral Proteins
0
NA protein, influenza A virus
EC 3.2.1.18
Neuraminidase
EC 3.2.1.18
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4763-4772Informations de copyright
© 2021 Wiley Periodicals LLC.
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