Atomistic insight on structure and dynamics of spinach acyl carrier protein with substrate length.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
07 09 2021
07 09 2021
Historique:
received:
11
08
2020
revised:
08
12
2020
accepted:
10
12
2020
pubmed:
26
2
2021
medline:
29
10
2021
entrez:
25
2
2021
Statut:
ppublish
Résumé
The plant acyl-acyl carrier protein (ACP) desaturases are a family of soluble enzymes that convert saturated fatty acyl-ACPs into their cis-monounsaturated equivalents in an oxygen-dependent reaction. These enzymes play a key role in biosynthesis of monounsaturated fatty acids in plants. ACPs are central proteins in fatty acid biosynthesis that deliver acyl chains to desaturases. They have been reported to show a varying degree of local dynamics and structural variability depending on the acyl chain size. It has been suggested that substrate-specific changes in ACP structure and dynamics have a crucial impact on the desaturase enzymatic activity. Using molecular dynamics simulations, we investigated the intrinsic solution structure and dynamics of ACP from spinach with four different acyl chains: capric (C
Identifiants
pubmed: 33631202
pii: S0006-3495(21)00161-2
doi: 10.1016/j.bpj.2020.12.036
pmc: PMC8456182
pii:
doi:
Substances chimiques
Acyl Carrier Protein
0
Fatty Acids
0
Fatty Acids, Monounsaturated
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3841-3853Informations de copyright
Copyright © 2021 Biophysical Society. All rights reserved.
Références
J Chem Theory Comput. 2008 Mar;4(3):435-47
pubmed: 26620784
Biochem J. 2020 Jan 31;477(2):491-508
pubmed: 31922183
J Lipid Res. 2003 Jan;44(1):1-10
pubmed: 12518017
Biochemistry. 2006 Apr 25;45(16):5217-27
pubmed: 16618110
J Biol Chem. 2008 Nov 28;283(48):33620-9
pubmed: 18809688
Nat Prod Rep. 2007 Aug;24(4):750-73
pubmed: 17653358
Methods Mol Biol. 2014;1084:193-226
pubmed: 24061923
J Mol Biol. 2007 Jan 5;365(1):135-45
pubmed: 17059829
Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:611-641
pubmed: 15012248
Plant J. 1999 Mar;17(6):679-88
pubmed: 10366274
Proc Natl Acad Sci U S A. 1997 May 13;94(10):4872-7
pubmed: 9144157
PLoS One. 2019 Jul 10;14(7):e0219435
pubmed: 31291335
Bioinformatics. 2013 Apr 1;29(7):845-54
pubmed: 23407358
Chembiochem. 2015 Mar 2;16(4):528-547
pubmed: 25676190
J Biol Chem. 2007 Jul 6;282(27):19863-71
pubmed: 17463003
Nat Prod Rep. 2012 Oct;29(10):1111-37
pubmed: 22930263
Methods Enzymol. 2011;487:545-74
pubmed: 21187238
Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):16594-9
pubmed: 21930947
Biochemistry. 2010 Jan 26;49(3):470-7
pubmed: 20014832
J Chem Theory Comput. 2015 Jul 14;11(7):3499-509
pubmed: 26190950
Angew Chem Int Ed Engl. 2019 Aug 5;58(32):10888-10892
pubmed: 31140212
J Mol Biol. 2009 Jan 16;385(2):381-92
pubmed: 19041878
Int J Mol Sci. 2020 Apr 09;21(7):
pubmed: 32283632
J Phys Chem B. 2015 Jul 23;119(29):9268-77
pubmed: 25402636
J Biol Chem. 2009 Jul 10;284(28):18559-63
pubmed: 19363032
J Chem Phys. 2016 Apr 7;144(13):130901
pubmed: 27059554
Methods Enzymol. 2009;459:395-433
pubmed: 19362649