Development of broad-spectrum and sustainable resistance in cotton against major insects through the combination of Bt and plant lectin genes.
ASAL
Cotton pests
Insecticidal crystal proteins
Vip3Aa
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
02
09
2020
accepted:
27
01
2021
pubmed:
27
2
2021
medline:
16
6
2021
entrez:
26
2
2021
Statut:
ppublish
Résumé
Second generation Bt insecticidal toxin in comibination with Allium sativum leaf agglutinin gene has been successfully expressed in cotton to develop sustainable resistance against major chewing and sucking insects. The first evidence of using the Second-generation Bt gene in combination with Allium sativum plant lectin to develop sustainable resistance against chewing and sucking insects has been successfully addressed in the current study. Excessive use of Bt δ-endotoxins in the field is delimiting its insecticidal potential. Second-generation Bt Vip3Aa could be the possible alternative because it does not share midgut receptor sites with any known cry proteins. Insecticidal potential of plant lectins against whitefly remains to be evaluated. In this study, codon-optimized synthetic Bt Vip3Aa gene under CaMV35S promoter and Allium sativum leaf agglutinin gene under phloem-specific promoter were transformed in a local cotton variety. Initial screening of putative transgenic cotton plants was done through amplification, histochemical staining and immunostrip assay. The mRNA expression of Vip3Aa gene was increased to be ninefold in transgenic cotton line L
Identifiants
pubmed: 33634360
doi: 10.1007/s00299-021-02669-6
pii: 10.1007/s00299-021-02669-6
doi:
Substances chimiques
Agglutinins
0
Bacterial Proteins
0
Plant Lectins
0
Vip3A protein, Bacillus thuringiensis
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
707-721Subventions
Organisme : Higher Education Commission, Pakistan
ID : 8243
Références
Abbas W, Rehman S, Rashid A, Kamran M, Atiq M, ul Haq ME (2020) Comparative efficacy of different plant extracts to manage the cotton leaf curl virus disease and its vector (Bemisia tabaci L.) Pakistan J Agricultural Res 33:22–26
Ahmad A et al (2017) Engineered disease resistance in cotton using RNA-interference to knock down cotton leaf curl Kokhran virus-Burewala and cotton leaf curl Multan betasatellite expression Viruses 9:257
Ahmed M, Shah A, Rauf M, Habib I, Shehzad K (2017) Ectopic expression of the Leptochloa fusca and Allium cepa lectin genes in tobacco plant for resistance against Mealybug (Phenacoccus solenopsis). J Genet Genomes 1:2
Ali A, Ahmed S, Nasir IA, Rao AQ, Ahmad S, Husnain T (2016) Evaluation of two cotton varieties CRSP1 and CRSP2 for genetic transformation efficiency, expression of transgenes Cry1Ac+ Cry2A. GT Gene Insect Mortality Biotechnol Rep 9:66–73
Azam S et al (2013) Dissemination of Bt cotton in cotton growing belt of Pakistan. Advancements Life Sci 1:18–26
Bajwa KS, Shahid AA, Rao AQ, Bashir A, Aftab A, Husnain T (2015) Stable transformation and expression of GhEXPA8 fiber expansin gene to improve fiber length and micronaire value in cotton. Front Plant Sci 6:838
pubmed: 26583018
pmcid: 4628126
Bakhsh K, Hassan I, Maqbool A (2005) Factors affecting cotton yield: a case study of Sargodha (Pakistan). J Agriculture Soc Sci 1:332–334
Bazargani MM, Tohidfar M, Ghareyazie B, Jouzani GS, Sayed-Tabatabaei BE, Golabchian R (2010) Transformation of Iranian cotton varieties using shoot apex. Transgenic Plant Res 3:107–112
Bett B et al (2017) Transgenic cowpeas (Vigna unguiculata L. Walp) expressing Bacillus thuringiensis Vip3Ba protein are protected against the Maruca pod borer (Maruca vitrata) Plant Cell. Tissue and Organ Culture (PCTOC) 131:335–345
Bravo A, Pacheco S, Gómez I, Garcia-Gómez B, Onofre J, Soberón M (2017) Insecticidal proteins from Bacillus thuringiensis and their mechanism of action. In: Bacillus thuringiensis and Lysinibacillus sphaericus. Springer, pp 53–66
Burkness EC, Dively G, Patton T, Morey AC, Hutchison WD (2010) Novel Vip3A Bacillus thuringiensis (Bt) maize approaches high-dose efficacy against Helicoverpa zea (Lepidoptera: Noctuidae) under field conditions: Implications for resistance management GM crops 1:337–343
Chandan K, Prasad BD, Ravi R, Tushar R, Nimmy M, Vinod K (2017) Transgenic plants and their application in crop improvement. In: Plant Biotechnology, Volume 2. Apple Academic Press, pp 141–172
Chandrasekhar K, Vijayalakshmi M, Vani K, Kaul T, Reddy MK (2014) Phloem-specific expression of the lectin gene from Allium sativum confers resistance to the sap-sucker Nilaparvata lugens. Biotech Lett 36:1059–1067
Chaudhry IS, Khan MB, Akhtar MH (2009) Economic analysis of competing crops with special reference to cotton production in Pakistan: the case of Multan and Bahawalpur regions. Pakistan J Soc Sci (PJSS) 29(1):51–63
Chen WB et al (2017) Transgenic cotton coexpressing Vip3A and Cry1Ac has a broad insecticidal spectrum against lepidopteran pests. J Invertebrate Pathol 149:59–65
Cohen MB, Gould F, Bentur JS (2000) Bt rice: practical steps to sustainable use. International Rice Res Note 25:4–10
de Oliveira RS et al. (2016) Transgenic cotton plants expressing Cry1Ia12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis) Frontiers in plant science 7:165
Estruch JJ, Warren GW, Mullins MA, Nye GJ, Craig JA, Koziel MG (1996) Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. Proc Natl Acad Sci 93:5389–5394
pubmed: 8643585
Farooq O (2016) Chapter 2: agriculture, economic survey of Pakistan. Economic Advisor’s Wing, Finance Division, Islamabad
Gul A et al (2020) Constitutive expression of asparaginase in Gossypium hirsutum triggers insecticidal activity against Bemisia tabaci. Sci Rep 10:1–11
Jaakola L, Pirttilä AM, Halonen M, Hohtola A (2001) Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit Molecular Biotechnology 19:201–203
Javaid S, Amin I, Jander G, Mukhtar Z, Saeed NA, Mansoor S (2016) A transgenic approach to control hemipteran insects by expressing insecticidal genes under phloem-specific promoters. Sci Rep 6:34706
pubmed: 27708374
pmcid: 5052614
Javaid S, Naz S, Amin I, Jander G, Ul-Haq Z, Mansoor S (2018) Computational and biological characterization of fusion proteins of two insecticidal proteins for control of insect pests. Scientific Rep 8:4837
Khan M, Mahmood HZ, Damalas CA (2015) Pesticide use and risk perceptions among farmers in the cotton belt of Punjab. Pakistan Crop Protection 67:184–190
Lee MK, Miles P, Chen JS (2006) Brush border membrane binding properties of Bacillus thuringiensis Vip3A toxin to Heliothis virescens and Helicoverpa zea midguts. Biochem Biophys Res Commun 339:1043–1047
pubmed: 16337146
Liu JG, Yang AZ, Shen XH, Hua BG, Shi GL (2011) Specific binding of activated Vip3Aa10 to Helicoverpa armigera brush border membrane vesicles results in pore formation. J Invertebrate Pathol 108:92–97
Ludgate Z (2013) Whitefly resistance monitoring 2010–2013 Queensland Department of Agriculture. Fish Forest
Macedo MLR, Oliveira CF, Oliveira CT (2015) Insecticidal activity of plant lectins and potential application in crop protection. Molecules 20:2014–2033
pubmed: 25633332
pmcid: 6272522
Majeed A, Husnain T, Riazuddin S (2000) Transformation of virus-resistant genotype of Gossypium hirsutum L. with pesticidal gene. Plant Biotechnol 17:105–110
Mansoor S, Bashir S, Zubair M (2020) Is Bt gene cotton adoption paying off farmers in Pakistan? Asian Dev Policy Rev 8:30–41
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
Prism G (2007) version 5.01 GraphPad Software Inc: San Diego, CA, USA
Puspito AN et al (2015) Transformation and evaluation of Cry1Ac+ Cry2A and GTGene in Gossypium hirsutum L. Front Plant Sci 6:943
pubmed: 26617613
pmcid: 4641916
Rani S, Sharma V, Hada A, Bhattacharya R, Koundal K (2017) Fusion gene construct preparation with lectin and protease inhibitor genes against aphids and efficient genetic transformation of Brassica juncea using cotyledons explants. Acta Physiol Plantarum 39:115
Rao AQ, Irfan M, Saleem Z, Nasir IA, Riazuddin S, Husnain T (2011) Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum). J Zhejiang Univ Sci B 12:326–334
pubmed: 21462389
pmcid: 3072596
Rashid B, Husnain T, Riazuddin S (2004) In vitro shoot tip culture of cotton (Gossypium hirsutum) Pakistan. J Bot 36:817–823
Raybould A, Vlachos D (2011) Non-target organism effects tests on Vip3A and their application to the ecological risk assessment for cultivation of MIR162 maize. Transgenic Res 20:599–611
pubmed: 20839052
Reisig DD et al (2018) Long-term empirical and observational evidence of practical Helicoverpa zea resistance to cotton with pyramided Bt toxins. J Econ Entomol 111(1824):1833
Romeis J et al (2008) Assessment of risk of insect-resistant transgenic crops to nontarget arthropods. Nat Biotechnol 26:203
pubmed: 18259178
Saini R (2011) Plant health diagnostics and loss assessment: an overview vice-chancellor CCS Haryana agricultural University HISAR-125 004 (Haryana) India:1
Sambrook J, Russell DW, Russell DW (2001) Molecular cloning: a laboratory manual (3-volume set) vol 999. Cold spring harbor laboratory press New York
Sarwar MB et al (2019) De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms. Sci Rep 9:396
pubmed: 30674899
pmcid: 6344536
Satyavathi V, Prasad V, Lakshmi BG, Sita GL (2002) High efficiency transformation protocol for three Indian cotton varieties via Agrobacterium tumefaciens. Plant Sci 162:215–223
Shah MA, Farooq M, Hussain M (2017) Evaluation of transplanting Bt cotton in a cotton–wheat cropping system. Exp Agriculture 53:227–241
Siddiqui HA et al (2019) Development and evaluation of double gene transgenic cotton lines expressing Cry toxins for protection against chewing insect pests. Sci Rep 9:11774
pubmed: 31409859
pmcid: 6692333
Song F, Lin Y, Chen C, Shao E, Guan X, Huang Z (2016) Insecticidal activity and histopathological effects of Vip3Aa protein from Bacillus thuringiensis on Spodoptera litura. J Microbiol Biotechnol 26(10):1774–1780
pubmed: 27435544
Sukumar S, Callahan F, Dollar D, Creech J (1997) Effect of Lyophilization of cotton tissue on quality of extractable DNA RNA, and protein. J Cotton Sci 1:10–14
Tabashnik BE, Carrière Y (2019) Global patterns of resistance to Bt crops highlighting pink bollworm in the United States, China, and India. J Econ Entomol
Tabashnik BE, Brévault T, Carrière Y (2013) Insect resistance to Bt crops: lessons from the first billion acres. Nat Biotechnol 31:510
Tsuchiya D, Taga M (2001) Cytological karyotyping of three Cochliobolus spp. by the germ tube burst method. Phytopathology 91:354–360
Vajhala CS, Sadumpati VK, Nunna HR, Puligundla SK, Vudem DR, Khareedu VR (2013) Development of transgenic cotton lines expressing Allium sativum agglutinin (ASAL) for enhanced resistance against major sap-sucking pests. PLoS ONE 8(9):e72542
pubmed: 24023750
pmcid: 3762794
Wamiq G, Akhtar S, Khan Z, Alam P. Khan J (2016) Development of an efficient method for regeneration and Agrobacterium mediated transformation of cotton (Gossypium hirsutum L.) cv. HS6 Indian. J. Biotechnol. 15: 39-47
Wojciechowska M, Stepnowski P, Gołębiowski M (2016) The use of insecticides to control insect pests. Invertebrate Survival J 13:210–220
Wu J, Luo X, Zhang X, Shi Y, Tian Y (2011) Development of insect-resistant transgenic cotton with chimeric TVip3A* accumulating in chloroplasts. Transgenic Res 20:963–973
pubmed: 21246398
Yarasi B, Sadumpati V, Immanni CP, Vudem DR, Khareedu VR (2008) Transgenic rice expressing Allium sativum leaf agglutinin (ASAL) exhibits high level resistance against major sap-sucking pests BMC. Plant Biol 8:102
Yasmeen A et al (2016) Amplicon-based RNA interference targeting V2 gene of cotton leaf curl Kokhran Virus-Burewala strain can provide resistance in transgenic cotton plants. Mol Biotechnol 58:807–820
pubmed: 27757798
pmcid: 5102983
Zhang BH, Guo TL, Wang QL (2000) Inheritance and segregation of exogenous genes in transgenic cotton. J Genet 79:71–75