Structural analysis and biochemical properties of laccase enzymes from two Pediococcus species.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
revised:
22
12
2020
received:
25
09
2020
accepted:
31
12
2020
pubmed:
27
2
2021
medline:
15
5
2021
entrez:
26
2
2021
Statut:
ppublish
Résumé
Prokaryotic laccases are emergent biocatalysts. However, they have not been broadly found and characterized in bacterial organisms, especially in lactic acid bacteria. Recently, a prokaryotic laccase from the lactic acid bacterium Pediococcus acidilactici 5930, which can degrade biogenic amines, was discovered. Thus, our study aimed to shed light on laccases from lactic acid bacteria focusing on two Pediococcus laccases, P. acidilactici 5930 and Pediococcus pentosaceus 4816, which have provided valuable information on their biochemical activities on redox mediators and biogenic amines. Both laccases are able to oxidize canonical substrates as ABTS, ferrocyanide and 2,6-DMP, and non-conventional substrates as biogenic amines. With ABTS as a substrate, they prefer an acidic environment and show sigmoidal kinetic activity, and are rather thermostable. Moreover, this study has provided the first structural view of two lactic acid bacteria laccases, revealing new structural features not seen before in other well-studied laccases, but which seem characteristic for this group of bacteria. We believe that understanding the role of laccases in lactic acid bacteria will have an impact on their biotechnological applications and provide a framework for the development of engineered lactic acid bacteria with enhanced properties.
Identifiants
pubmed: 33635570
doi: 10.1111/1751-7915.13751
pmc: PMC8085982
doi:
Substances chimiques
Laccase
EC 1.10.3.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1026-1043Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM111244
Pays : United States
Informations de copyright
© 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
Références
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Cell Mol Life Sci. 2015 Mar;72(5):857-68
pubmed: 25586561
J Biol Chem. 2003 May 23;278(21):19416-25
pubmed: 12637519
Methods Mol Biol. 2014;1079:245-62
pubmed: 24170407
Appl Microbiol Biotechnol. 2015 Dec;99(23):9987-99
pubmed: 26227413
Environ Sci Process Impacts. 2015 Feb;17(2):326-42
pubmed: 25590782
Cell Mol Life Sci. 2015 Mar;72(5):911-22
pubmed: 25572294
Appl Biochem Biotechnol. 2009 Aug;158(2):416-31
pubmed: 18931956
Front Microbiol. 2016 Apr 08;7:482
pubmed: 27092133
Bioresour Technol. 2017 Apr;230:67-75
pubmed: 28161622
Metallomics. 2015 May;7(5):776-85
pubmed: 25679350
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Mol Biotechnol. 2016 Dec;58(12):789-800
pubmed: 27771851
3 Biotech. 2017 Oct;7(5):323
pubmed: 28955620
Angew Chem Int Ed Engl. 2012 Feb 20;51(8):1861-4
pubmed: 22250058
Trends Biotechnol. 2000 Feb;18(2):41-2
pubmed: 10652504
Microb Cell Fact. 2008 Nov 20;7:32
pubmed: 19019256
Appl Biochem Biotechnol. 1997 Jun;66(3):197-238
pubmed: 9276922
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
PLoS One. 2017 Apr 6;12(4):e0175144
pubmed: 28384218
Appl Microbiol Biotechnol. 2014 Jan;98(1):185-98
pubmed: 23515835
FEBS J. 2009 Nov;276(21):6285-300
pubmed: 19780817
J Appl Microbiol. 2016 Nov;121(5):1335-1345
pubmed: 27451019
Crit Rev Biotechnol. 2018 Mar;38(2):294-307
pubmed: 28738694
PLoS One. 2017 Oct 11;12(10):e0186019
pubmed: 29020076
Cell Mol Life Sci. 2015 Mar;72(5):897-910
pubmed: 25586560
Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):772-9
pubmed: 24598746
PLoS One. 2015 Mar 19;10(3):e0119833
pubmed: 25790466
Dalton Trans. 2012 May 28;41(20):6247-55
pubmed: 22481612
BMC Biotechnol. 2011 Jan 25;11:9
pubmed: 21266052
J Agric Food Chem. 2011 Sep 14;59(17):9290-302
pubmed: 21790191
Bioresour Technol. 2013 Apr;134:81-6
pubmed: 23500563
Appl Microbiol Biotechnol. 2012 Mar;93(5):1817-30
pubmed: 22314515
Protein Sci. 2004 Sep;13(9):2388-97
pubmed: 15295117
Nat Struct Biol. 2002 Aug;9(8):601-5
pubmed: 12118243
Dalton Trans. 2010 Mar 21;39(11):2875-82
pubmed: 20200715
Comb Chem High Throughput Screen. 2008 Dec;11(10):807-16
pubmed: 19075602
J Biol Chem. 2004 May 28;279(22):23472-6
pubmed: 14764581
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Cell Mol Life Sci. 2018 Oct;75(19):3569-3592
pubmed: 30046841
Bioorg Chem. 2012 Aug;43:44-50
pubmed: 22137502
J Biol Chem. 2011 Oct 28;286(43):37849-57
pubmed: 21903583
PLoS One. 2013 Jun 03;8(6):e65633
pubmed: 23755261
Database (Oxford). 2011 Apr 15;2011:bar006
pubmed: 21498547
Acta Crystallogr F Struct Biol Commun. 2016 Apr;72(Pt 4):328-35
pubmed: 27050268
Methods. 2011 Sep;55(1):81-93
pubmed: 21763424
Protein Sci. 2011 Jan;20(1):6-18
pubmed: 20981744
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
J Biol Chem. 2002 May 24;277(21):18849-59
pubmed: 11884407
Sheng Wu Gong Cheng Xue Bao. 2019 Jul 25;35(7):1286-1294
pubmed: 31328485
Appl Microbiol Biotechnol. 2016 Apr;100(7):3113-24
pubmed: 26590586
J Ind Microbiol Biotechnol. 2010 Aug;37(8):863-9
pubmed: 20473548
Extremophiles. 2017 May;21(3):563-571
pubmed: 28314922
PLoS One. 2018 Nov 5;13(11):e0206589
pubmed: 30395580
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Sci Rep. 2018 Nov 23;8(1):17285
pubmed: 30470810
Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2766-71
pubmed: 11867755
PLoS One. 2011;6(10):e25724
pubmed: 22022440