Epidemiology of molecular probes in Xpert MTB/RIF assay in Khyber Pakhtunkhwa, Pakistan.
Antibiotics, Antitubercular
/ therapeutic use
Codon
/ genetics
Drug Resistance, Multiple, Bacterial
/ genetics
Gold
Humans
Metal Nanoparticles
/ chemistry
Molecular Probes
/ chemistry
Mutation
/ genetics
Mycobacterium tuberculosis
/ drug effects
Pakistan
Rifampin
/ therapeutic use
Sensitivity and Specificity
Tuberculosis, Multidrug-Resistant
/ diagnosis
Tuberculosis, Pulmonary
/ diagnosis
AuNPs
Probe
RRDR
Xpert MTB/RIF assay
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
28
07
2020
accepted:
13
02
2021
revised:
24
01
2021
pubmed:
1
3
2021
medline:
30
6
2021
entrez:
28
2
2021
Statut:
ppublish
Résumé
Regardless of a plethora of advanced diagnostics, TB and drug resistance remains a principal killer. We proposed gold nanoparticles (AuNPs) attached with probes to enhance the efficiency of GeneXpert MTB/RIF assay instead of conventional dye probes for molecular detection. A total of 15,000 samples were collected from TB suspects and subjected to Xpert MTB/RIF assay, where 6800 (45.3%) were detected as MTB positive, 280 (4.3%) were detected to harbor mutations in the RRDR, while invalid /errors were found in 690 (4.6%) cases. The mutations were detected by probe E, 199 (71.1%), while probes B and D, 30 and 26 (10% and 9%), respectively. In the Xpert MTB/RIF Assay were found mutations picked by probes E and B codons 529-533 (71%) and 512-518 (10%), respectively. The fast-rising works of TB nano-diagnostics, of Xpert probes, may improve by the applications of gold nanoparticle probes.
Identifiants
pubmed: 33640990
doi: 10.1007/s00203-021-02242-5
pii: 10.1007/s00203-021-02242-5
doi:
Substances chimiques
Antibiotics, Antitubercular
0
Codon
0
Molecular Probes
0
Gold
7440-57-5
Rifampin
VJT6J7R4TR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2249-2256Références
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