Proteome-minimized outer membrane vesicles from
Animals
Antigens, Bacterial
/ immunology
Bacterial Outer Membrane
/ immunology
Bacterial Outer Membrane Proteins
/ metabolism
Bacterial Vaccines
Biological Transport
CD8-Positive T-Lymphocytes
/ immunology
Escherichia coli
/ immunology
Escherichia coli Infections
/ immunology
Extracellular Vesicles
/ immunology
Humans
Interleukin-6
/ metabolism
Mice
Proteome
/ metabolism
Recombinant Proteins
/ immunology
Synthetic Biology
/ methods
Toll-Like Receptor 2
/ metabolism
Vaccine Development
/ methods
cancer
infectious diseases
outer membrane vesicles (OMVs)
synthetic biology
vaccines
Journal
Journal of extracellular vesicles
ISSN: 2001-3078
Titre abrégé: J Extracell Vesicles
Pays: United States
ID NLM: 101610479
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
28
05
2020
revised:
26
12
2020
accepted:
13
01
2021
entrez:
1
3
2021
pubmed:
2
3
2021
medline:
2
3
2021
Statut:
ppublish
Résumé
Because of their potent adjuvanticity, ease of manipulation and simplicity of production Gram-negative Outer Membrane Vesicles OMVs have the potential to become a highly effective vaccine platform. However, some optimization is required, including the reduction of the number of endogenous proteins, the increase of the loading capacity with respect to heterologous antigens, the enhancement of productivity in terms of number of vesicles per culture volume. In this work we describe the use of Synthetic Biology to create
Identifiants
pubmed: 33643549
doi: 10.1002/jev2.12066
pii: JEV212066
pmc: PMC7886703
doi:
Substances chimiques
Antigens, Bacterial
0
Bacterial Outer Membrane Proteins
0
Bacterial Vaccines
0
Interleukin-6
0
Proteome
0
Recombinant Proteins
0
Toll-Like Receptor 2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
e12066Informations de copyright
© 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
Déclaration de conflit d'intérêts
Guido Grandi, Alberto Grandi, Enrico König, Ilaria Zanella, Laura Fantappiè and Carmela Irene are co‐inventors of patents on OMVs; Alberto Grandi and Guido Grandi are involved in a biotech company interested in exploiting the OMV platform.
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