Investigation of the domain line tension in asymmetric vesicles prepared via hemifusion.
Asymmetric bilayers
Asymmetric giant unilamellar vesicles
Coexistence of liquid disordered and liquid ordered phases
Domain line tension
Hemifusion
Journal
Biochimica et biophysica acta. Biomembranes
ISSN: 1879-2642
Titre abrégé: Biochim Biophys Acta Biomembr
Pays: Netherlands
ID NLM: 101731713
Informations de publication
Date de publication:
01 06 2021
01 06 2021
Historique:
received:
30
09
2020
revised:
28
01
2021
accepted:
12
02
2021
pubmed:
2
3
2021
medline:
9
9
2021
entrez:
1
3
2021
Statut:
ppublish
Résumé
The plasma membrane (PM) is asymmetric in lipid composition. The distinct and characteristic lipid compositions of the exoplasmic and cytoplasmic leaflets lead to different lipid-lipid interactions and physical-chemical properties in each leaflet. The exoplasmic leaflet possesses an intrinsic ability to form coexisting ordered and disordered fluid domains, whereas the cytoplasmic leaflet seems to form a single fluid phase. To better understand the interleaflet interactions that influence domains, we compared asymmetric model membranes that capture salient properties of the PM with simpler symmetric membranes. Using asymmetric giant unilamellar vesicles (aGUVs) prepared by hemifusion with a supported lipid bilayer, we investigate the domain line tension that characterizes the behavior of coexisting ordered + disordered domains. The line tension can be related to the contact perimeter of the different phases. Compared to macroscopic phase separation, the appearance of modulated phases was found to be a robust indicator of a decrease in domain line tension. Symmetric GUVs of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)/1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC)/cholesterol (chol) were formed into aGUVs by replacing the GUV outer leaflet with DOPC/chol = 0.8/0.2 in order to create a cytoplasmic leaflet model. These aGUVs revealed lower line tension for the ordered + disordered domains of the exoplasmic model leaflet.
Identifiants
pubmed: 33647248
pii: S0005-2736(21)00037-7
doi: 10.1016/j.bbamem.2021.183586
pmc: PMC8606045
mid: NIHMS1684440
pii:
doi:
Substances chimiques
Phosphatidylcholines
0
Unilamellar Liposomes
0
Cholesterol
97C5T2UQ7J
1,2-distearoyllecithin
EAG959U971
1,2-oleoylphosphatidylcholine
EDS2L3ODLV
1-palmitoyl-2-oleoylphosphatidylcholine
TE895536Y5
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
183586Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM105684
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Références
Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17367-72
pubmed: 18987307
Langmuir. 2016 May 24;32(20):5195-200
pubmed: 27128636
Biophys J. 2001 Jun;80(6):2775-88
pubmed: 11371452
Biophys J. 2019 Jun 18;116(12):2356-2366
pubmed: 31023537
Nat Chem Biol. 2020 Jun;16(6):644-652
pubmed: 32367017
J Am Chem Soc. 2011 May 4;133(17):6563-77
pubmed: 21473645
Biophys J. 2011 Jan 5;100(1):L1-3
pubmed: 21190650
Biochim Biophys Acta. 1999 Mar 4;1417(2):232-45
pubmed: 10082799
Biochim Biophys Acta Biomembr. 2019 Jun 1;1861(6):1112-1122
pubmed: 30904407
Biochim Biophys Acta. 2013 Sep;1828(9):2204-14
pubmed: 23747294
Biophys J. 2018 Apr 24;114(8):1921-1935
pubmed: 29694869
Biophys J. 2007 Dec 15;93(12):4268-77
pubmed: 17766349
Proc Natl Acad Sci U S A. 2020 Aug 18;117(33):19943-19952
pubmed: 32759206
Biophys J. 2009 Dec 16;97(12):3113-22
pubmed: 20006948
J Biol Chem. 2009 Mar 6;284(10):6079-92
pubmed: 19129198
Nat Rev Mol Cell Biol. 2008 Feb;9(2):112-24
pubmed: 18216768
Biochim Biophys Acta. 2010 Jul;1798(7):1324-32
pubmed: 20302841
Biophys J. 2016 Mar 8;110(5):1110-24
pubmed: 26958888
Biophys J. 2007 Nov 1;93(9):3169-81
pubmed: 17644560
Biochim Biophys Acta. 2012 Mar;1818(3):402-10
pubmed: 22047743
Biophys J. 2011 Nov 16;101(10):2417-25
pubmed: 22098740
Nat Protoc. 2018 Sep;13(9):2086-2101
pubmed: 30190552
Biophys J. 2018 Jan 9;114(1):146-157
pubmed: 29320681
Biophys J. 2018 Aug 21;115(4):664-678
pubmed: 30082033
J Am Chem Soc. 2013 May 8;135(18):6853-9
pubmed: 23391155
Biophys J. 2002 Oct;83(4):2118-25
pubmed: 12324429
Biochim Biophys Acta. 2005 Dec 30;1746(3):186-92
pubmed: 15992943
Langmuir. 2017 Apr 18;33(15):3731-3741
pubmed: 28106399
J Phys Chem Lett. 2020 Jul 2;11(13):5171-5176
pubmed: 32515980
Biophys J. 2008 Mar 1;94(5):L32-4
pubmed: 18096628
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10718-21
pubmed: 12963816
Biophys J. 2010 Sep 22;99(6):L44-6
pubmed: 20858410
Nat Commun. 2016 Apr 26;7:11401
pubmed: 27113279
Biophys J. 2011 Jul 20;101(2):L8-10
pubmed: 21767476
J Phys Chem B. 2020 Jun 18;124(24):4949-4959
pubmed: 32436388
Biophys J. 2017 Apr 11;112(7):1431-1443
pubmed: 28402885
Biophys J. 2020 Feb 4;118(3):624-642
pubmed: 31954503
J Am Chem Soc. 2016 Dec 28;138(51):16737-16744
pubmed: 27977192
Nat Commun. 2015 May 26;6:7238
pubmed: 26006266
Phys Rev Lett. 2012 Apr 27;108(17):178101
pubmed: 22680906
Biophys J. 2019 Sep 17;117(6):1037-1050
pubmed: 31493862
Nat Chem Biol. 2006 Nov;2(11):560-3
pubmed: 17051225
Biochim Biophys Acta. 2013 Apr;1828(4):1302-13
pubmed: 23337475
Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):124-8
pubmed: 18172219
Biophys J. 2011 Feb 16;100(4):996-1004
pubmed: 21320444
J Mol Biol. 2016 Dec 4;428(24 Pt A):4749-4764
pubmed: 27575334
Biophys J. 2001 Oct;81(4):2257-67
pubmed: 11566796
Nature. 2009 Feb 26;457(7233):1159-62
pubmed: 19098897