Trophectoderm biopsy protocols may impact the rate of mosaic blastocysts in cycles with pre-implantation genetic testing for aneuploidy.
Blastocyst biopsy protocol
Mosaicism
Next-generation sequencing
Pre-implantation genetic testing for aneuploidies (PGT-A)
Journal
Journal of assisted reproduction and genetics
ISSN: 1573-7330
Titre abrégé: J Assist Reprod Genet
Pays: Netherlands
ID NLM: 9206495
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
21
12
2020
accepted:
25
02
2021
pubmed:
5
3
2021
medline:
9
9
2021
entrez:
4
3
2021
Statut:
ppublish
Résumé
This study aimed to analyze the impact of different biopsy protocols on the rate of mosaic blastocysts. This is a retrospective cohort study which included 115 cycles with pre-implantation genetic testing for aneuploidy (PGT-A). Two groups were allocated based on the biopsy protocols: method 1 group, the zona pellucida (ZP) was drilled on day 3 embryos followed by trophectoderm (TE) biopsy; and method 2 group, the ZP was opened on day 5 or 6 blastocysts followed by TE biopsy. All biopsy samples were assessed using next-generation sequencing (NGS) at a single reference laboratory. The euploid, aneuploid, and mosaic blastocyst rates and clinical outcomes were compared. The mosaicism rate in the method 1 group was 19.58%, significantly higher than the method 2 group (8.12%; P < 0.05). No statistically significant difference was observed in euploid, aneuploid blastocyst rates, and clinical pregnancy rates between the two groups. Logistic regression analysis indicated that the biopsy protocols were independently associated with the mosaicism rates among all the variables. The present study showed that different biopsy protocols may have an impact on the mosaic blastocyst rate. ZP opening on day 3 combined with TE biopsy might increase the incidence of mosaic blastocysts.
Identifiants
pubmed: 33660205
doi: 10.1007/s10815-021-02137-w
pii: 10.1007/s10815-021-02137-w
pmc: PMC7929899
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1153-1162Subventions
Organisme : Chongqing Municipal Public Health Bureau, Chongqing People's Municipal Government
ID : 2019MSXM067
Organisme : Chongqing Municipal Public Health Bureau, Chongqing People's Municipal Government
ID : 2020MSXM086
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