HP1β carries an acidic linker domain and requires H3K9me3 for phase separation.
Phase separation
chromatin structure
heterochromatin
heterochromatin binding protein HP1
histone posttranslational modification
Journal
Nucleus (Austin, Tex.)
ISSN: 1949-1042
Titre abrégé: Nucleus
Pays: United States
ID NLM: 101518322
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
entrez:
4
3
2021
pubmed:
5
3
2021
medline:
14
1
2022
Statut:
ppublish
Résumé
Liquid-liquid phase separation (LLPS) mediated formation of membraneless organelles has been proposed to coordinate biological processes in space and time. Previously, the formation of phase-separated droplets was described as a unique property of HP1α. Here, we demonstrate that the positive net charge of the intrinsically disordered hinge region (IDR-H) of HP1 proteins is critical for phase separation and that the exchange of four acidic amino acids is sufficient to confer LLPS properties to HP1β. Surprisingly, the addition of mono-nucleosomes promoted H3K9me3-dependent LLPS of HP1β which could be specifically disrupted with methylated but not acetylated H3K9 peptides. HP1β mutants defective in H3K9me3 binding were less efficient in phase separation
Identifiants
pubmed: 33660589
doi: 10.1080/19491034.2021.1889858
pmc: PMC7939559
doi:
Substances chimiques
Chromosomal Proteins, Non-Histone
0
Heterochromatin
0
Histones
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
44-57Références
Proc Natl Acad Sci U S A. 2015 May 19;112(20):E2620-9
pubmed: 25941378
Biophys J. 2018 May 22;114(10):2262-2270
pubmed: 29628210
Nature. 2017 Jul 13;547(7662):241-245
pubmed: 28636597
Nature. 2019 Nov;575(7782):390-394
pubmed: 31618757
Nature. 2017 Jul 13;547(7662):236-240
pubmed: 28636604
Nat Commun. 2016 Apr 19;7:11310
pubmed: 27090491
Science. 2017 Sep 22;357(6357):
pubmed: 28935776
Nature. 2001 Mar 1;410(6824):120-4
pubmed: 11242054
Mol Cell. 2018 Feb 1;69(3):385-397.e8
pubmed: 29336876
Epigenetics. 2017 Feb;12(2):166-175
pubmed: 28059589
Cell. 2016 May 19;165(5):1067-1079
pubmed: 27203111
J Cell Sci. 2011 Jun 1;124(Pt 11):1878-90
pubmed: 21576353
PLoS One. 2012;7(10):e47848
pubmed: 23112857
Nat Genet. 2011 Mar;43(3):220-7
pubmed: 21317888
Curr Opin Genet Dev. 2000 Apr;10(2):204-10
pubmed: 10753776
PLoS Genet. 2009 Dec;5(12):e1000769
pubmed: 20011120
Mol Cell. 2020 Apr 16;78(2):236-249.e7
pubmed: 32101700
Science. 2001 Apr 6;292(5514):110-3
pubmed: 11283354
Mol Syst Biol. 2014 Aug 18;10:746
pubmed: 25134515
Chromosome Res. 2013 Aug;21(5):535-54
pubmed: 23996328
Nature. 2013 Apr 18;496(7445):377-81
pubmed: 23485968
Cell. 2019 Oct 3;179(2):470-484.e21
pubmed: 31543265
Cell Rep. 2017 Nov 21;21(8):2048-2057
pubmed: 29166597
J Cell Biol. 2005 Jun 6;169(5):733-43
pubmed: 15939760
Science. 2009 Jun 26;324(5935):1729-32
pubmed: 19460965
Biophys J. 2009 Dec 2;97(11):2876-85
pubmed: 19948116
Mol Cell. 2016 Jul 7;63(1):72-85
pubmed: 27392146
Cell. 2012 May 11;149(4):753-67
pubmed: 22579281
Annu Rev Cell Dev Biol. 2014;30:39-58
pubmed: 25288112
Cell. 2018 Jul 26;174(3):688-699.e16
pubmed: 29961577
Cell. 2009 Apr 3;137(1):146-58
pubmed: 19345193
Trends Cell Biol. 2018 Jun;28(6):420-435
pubmed: 29602697
Science. 2002 Mar 15;295(5562):2080-3
pubmed: 11859155
Trends Cell Biol. 2016 Jul;26(7):547-558
pubmed: 27051975
Biophys J. 2020 Feb 4;118(3):753-764
pubmed: 31952807
Dev Cell. 2010 Oct 19;19(4):625-38
pubmed: 20951352
Proc Natl Acad Sci U S A. 2018 Nov 20;115(47):11964-11969
pubmed: 30301810
Mol Cell. 2019 Nov 21;76(4):646-659.e6
pubmed: 31543422
Nat Rev Mol Cell Biol. 2015 Jan;16(1):18-29
pubmed: 25531225
Proc Natl Acad Sci U S A. 2002 Dec 10;99 Suppl 4:16462-9
pubmed: 12151603
Mol Cell. 2001 Apr;7(4):729-39
pubmed: 11336697
Nat Protoc. 2007;2(6):1445-57
pubmed: 17545981
Nucleic Acids Res. 2015 Sep 30;43(17):e112
pubmed: 26007658