The function of BAP18 on modulation of androgen receptor action in luteinized granulosa cells from normal weight women with and without PCOS.
Androgen receptor
BAP18
CYP19A1
Epigenetic
Polycystic ovary syndrome
Transcriptional coactivator
Journal
Molecular and cellular endocrinology
ISSN: 1872-8057
Titre abrégé: Mol Cell Endocrinol
Pays: Ireland
ID NLM: 7500844
Informations de publication
Date de publication:
01 05 2021
01 05 2021
Historique:
received:
09
11
2020
revised:
22
02
2021
accepted:
25
02
2021
pubmed:
5
3
2021
medline:
23
11
2021
entrez:
4
3
2021
Statut:
ppublish
Résumé
Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in reproductive-age women. In this study, BPTF associated protein of 18 kDa (BAP18) is decreased in luteinized granulosa cells (GCs) from PCOS women. BAP18 depletion significantly decreases CYP19A1 expression levels, leading to an abrogation in transfer capacity of androgen to estrogen in GCs. Also, BAP18 knockdown delays cell cycle G1 to S phase transition and induces cell apoptosis to decrease GCs proliferation. We also provide evidence showing BAP18 interacts with androgen receptor (AR) and enhances AR-mediated transactivation in GCs. Results indicate that AR or BAP18 recruits to androgen response elements (AREs) of CYP19A1 and FSHR, which are putative AR-induced genes in GCs. BAP18 interacts with Sp1 transcription factor and co-recruits to the promoter region of AR gene, resulting in AR transactivation in GCs. Taken together, these data provide new insights on the pathophysiology of PCOS.
Identifiants
pubmed: 33662476
pii: S0303-7207(21)00072-1
doi: 10.1016/j.mce.2021.111228
pii:
doi:
Substances chimiques
C17orf49 protein, human
0
DNA-Binding Proteins
0
Receptors, Androgen
0
Sp1 Transcription Factor
0
SP1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111228Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.