CRISPR-Cas12-based nucleic acids detection systems.

CRISPR-Cas Systems / genetics DNA / genetics DNA, Single-Stranded Gene Editing / methods
CRISPR CRISPR diagnostics Cas12 HOLMES Trans-cleavage

Journal

Methods (San Diego, Calif.)
ISSN: 1095-9130
Titre abrégé: Methods
Pays: United States
ID NLM: 9426302

Informations de publication

Date de publication:
07 2022
Historique:
received: 27 11 2020
revised: 19 01 2021
accepted: 07 02 2021
pubmed: 5 3 2021
medline: 22 6 2022
entrez: 4 3 2021
Statut: ppublish

Résumé

Because of the outstanding contribution in genome editing, CRISPR has undoubtedly become the most popular technology around the world and two pioneers are awarded the Nobel Prize in Chemistry this year. Besides, along with the discovery of nonspecific trans-cleavage activities of several Cas proteins such as Cas12 and Cas13, many CRISPR-based molecular diagnostic systems have been successfully created, showing advantages in sensitivity, specificity and operation convenience. Among them, systems with Cas12, which targets DNA and trans-cleaves single-stranded DNA probes, are both simple and highly efficient. Here in this review, we mainly focus on the Cas12-based methods and briefly discuss their applications in nucleic acids detection and beyond.

Identifiants

DOI: 10.1016/j.ymeth.2021.02.018 PMID: 33662563
pubmed: 33662563
pii: S1046-2023(21)00063-3
doi: 10.1016/j.ymeth.2021.02.018
pii:
doi:

Substances chimiques

DNA, Single-Stranded 0
DNA 9007-49-2

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

276-281

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Ross Ka-Kit Leung (RK)

Dongguan Maternal and Child Health Care Hospital/Dongguan Institute of Reproductive and Genetic Research, Dongguan 523120, China; Stanley Ho Centre for Emerging Infectious Diseases, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.

Qiu-Xiang Cheng (QX)

Tolo Biotechnology Company Limited, Shanghai 200233, China.

Zhi-Le Wu (ZL)

College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.

Gulfaraz Khan (G)

Department of Medical Microbiology and Immunology, College of Medicine & Health Sciences, United Arab Emirates University, United Arab Emirates.

Yang Liu (Y)

Shanghai Institute of Quality Inspection and Technical Research/National Quality Supervision and Inspection Center for Food Products (Shanghai), Shanghai 200233, China.

Hai-Yang Xia (HY)

Institute of Biopharmaceuticals, Taizhou University, Taizhou 318000, China.

Jin Wang (J)

Department of Clinical Laboratory, Shenzhen Second People's Hospital & Institute of Translational Medicine the First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen 518035, China. Electronic address: wangj01@hotmail.com.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › CRISPR-Cas12-based nucleic acids detection systems.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › CRISPR-Cas12-based nucleic acids detection systems.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › ADN simple brin › CRISPR-Cas12-based nucleic acids detection systems.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › CRISPR-Cas12-based nucleic acids detection systems.