Bacterial inactivation via microfluidic electroporation device with insulating micropillars.
Bacteria
Electroporation
Inactivation
Microfluidics
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
revised:
14
02
2021
received:
01
11
2020
accepted:
16
02
2021
pubmed:
6
3
2021
medline:
10
2
2022
entrez:
5
3
2021
Statut:
ppublish
Résumé
Electroporation is a promising method to inactivate cells and it has wide applications in medical science, biology and environmental health. Here, we investigate the bacteria inactivation performance of two different microfluidic electroporation devices with rhombus and circular micropillars used for generating locally enhanced electric field strength. Experiments are carried out to characterize the inactivation performance (i.e., the log removal efficiency) of two types of bacteria: Escherichia coli (E. coli, gram-negative) and Enterococcus faecalis (E. faecalis, gram-positive) in these two microfluidic devices. We find that under the same applied electric field, the device with rhombus micropillars performs better than the device with circular micropillars for both E. coli and E. faecalis. Numerical simulations show that due to the corner-induced singularity effect, the maximum electric field enhancement is higher in the device with rhombus micropillars than that in the device with circular micropillars. We also study the effects of DC and AC electric fields and flowrate. Our experiments demonstrate that the use of the DC field achieves higher log removal efficiencies than the use of AC field.
Identifiants
pubmed: 33665842
doi: 10.1002/elps.202000326
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1093-1101Informations de copyright
© 2021 Wiley-VCH GmbH.
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