Structure of membrane diacylglycerol kinase in lipid bilayers.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
05 03 2021
05 03 2021
Historique:
received:
15
01
2020
accepted:
05
02
2021
entrez:
6
3
2021
pubmed:
7
3
2021
medline:
11
8
2021
Statut:
epublish
Résumé
Diacylglycerol kinase (DgkA) is a small integral membrane protein, responsible for the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid. Its structures reported in previous studies, determined in detergent micelles by solution NMR and in monoolein cubic phase by X-ray crystallography, differ significantly. These differences point to the need to validate these detergent-based structures in phospholipid bilayers. Here, we present a well-defined homo-trimeric structure of DgkA in phospholipid bilayers determined by magic angle spinning solid-state NMR (ssNMR) spectroscopy, using an approach combining intra-, inter-molecular paramagnetic relaxation enhancement (PRE)-derived distance restraints and CS-Rosetta calculations. The DgkA structure determined in lipid bilayers is different from the solution NMR structure. In addition, although ssNMR structure of DgkA shows a global folding similar to that determined by X-ray, these two structures differ in monomeric symmetry and dynamics. A comparative analysis of DgkA structures determined in three different detergent/lipid environments provides a meaningful demonstration of the influence of membrane mimetic environments on the structure and dynamics of membrane proteins.
Identifiants
pubmed: 33674677
doi: 10.1038/s42003-021-01802-1
pii: 10.1038/s42003-021-01802-1
pmc: PMC7935881
doi:
Substances chimiques
Detergents
0
Lipid Bilayers
0
Phospholipids
0
Diacylglycerol Kinase
EC 2.7.1.107
Types de publication
Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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