Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process.
Cell Membrane
/ metabolism
Endosomes
/ metabolism
Host-Pathogen Interactions
/ physiology
Phytophthora infestans
/ physiology
Plant Diseases
/ microbiology
Plant Proteins
/ metabolism
Protein Interaction Maps
Proteins
/ metabolism
SNARE Proteins
/ metabolism
Nicotiana
/ metabolism
Transport Vesicles
/ metabolism
Journal
The Plant cell
ISSN: 1532-298X
Titre abrégé: Plant Cell
Pays: England
ID NLM: 9208688
Informations de publication
Date de publication:
02 07 2021
02 07 2021
Historique:
received:
01
10
2020
accepted:
19
02
2021
pubmed:
8
3
2021
medline:
2
9
2021
entrez:
7
3
2021
Statut:
ppublish
Résumé
Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically function by associating with host molecules and modulating their activities. This study aimed to identify the host processes targeted by the RXLR class of host-translocated effectors of the potato blight pathogen Phytophthora infestans. To this end, we performed an in planta protein-protein interaction screen by transiently expressing P. infestans RXLR effectors in Nicotiana benthamiana leaves followed by coimmunoprecipitation and liquid chromatography-tandem mass spectrometry. This screen generated an effector-host protein interactome matrix of 59 P. infestans RXLR effectors x 586 N. benthamiana proteins. Classification of the host interactors into putative functional categories revealed over 35 biological processes possibly targeted by P. infestans. We further characterized the PexRD12/31 family of RXLR-WY effectors, which associate and colocalize with components of the vesicle trafficking machinery. One member of this family, PexRD31, increased the number of FYVE positive vesicles in N. benthamiana cells. FYVE positive vesicles also accumulated in leaf cells near P. infestans hyphae, indicating that the pathogen may enhance endosomal trafficking during infection. This interactome dataset will serve as a useful resource for functional studies of P. infestans effectors and of effector-targeted host processes.
Identifiants
pubmed: 33677602
pii: 6156641
doi: 10.1093/plcell/koab069
pmc: PMC8254500
doi:
Substances chimiques
Plant Proteins
0
Proteins
0
SNARE Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1447-1471Subventions
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.
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