Isolation and genetic characterization of SARS-CoV-2 from Indian patients in a single family without H/O travel abroad.
Animals
COVID-19
/ virology
COVID-19 Nucleic Acid Testing
Chlorocebus aethiops
Coronavirus Papain-Like Proteases
/ genetics
Exonucleases
/ genetics
Genome, Viral
Humans
India
Mutation
Nasopharynx
/ virology
Phylogeny
RNA-Dependent RNA Polymerase
/ genetics
SARS-CoV-2
/ genetics
Spike Glycoprotein, Coronavirus
/ genetics
Travel
Vero Cells
Viral Nonstructural Proteins
/ genetics
Viral Plaque Assay
Whole Genome Sequencing
Mutation
Phylogeny
Plaque assay
SARS-CoV-2
TCID50
Virus isolates
Journal
Virus genes
ISSN: 1572-994X
Titre abrégé: Virus Genes
Pays: United States
ID NLM: 8803967
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
16
06
2020
accepted:
18
01
2021
pubmed:
9
3
2021
medline:
5
6
2021
entrez:
8
3
2021
Statut:
ppublish
Résumé
In view of the rapidly progressing COVID-19 pandemic, our aim was to isolate and characterize SARS-CoV-2 from Indian patients. SARS-CoV-2 was isolated from nasopharyngeal swabs collected from the two members of a family without any history of (H/O) travel abroad. Both the virus isolates (8003 and 8004) showed CPE on day 3 post-inoculation, viral antigens by immunofluorescence assay and produced distinct, clear and uniform plaques. Infectious virus titers were 5 × 10
Identifiants
pubmed: 33683658
doi: 10.1007/s11262-021-01826-z
pii: 10.1007/s11262-021-01826-z
pmc: PMC7938031
doi:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
Viral Nonstructural Proteins
0
spike protein, SARS-CoV-2
0
Nsp3 protein, SARS-CoV
EC 2.7.7.48
RNA-Dependent RNA Polymerase
EC 2.7.7.48
Exonucleases
EC 3.1.-
Coronavirus Papain-Like Proteases
EC 3.4.22.2
papain-like protease, SARS-CoV-2
EC 3.4.22.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
245-249Subventions
Organisme : Department of Biotechnology, Govt. of India (IN)
ID : BT/NBM0095/02/18
Références
Med J Aust. 2020 Jun;212(10):459-462
pubmed: 32237278
Emerg Microbes Infect. 2020 Dec;9(1):837-842
pubmed: 32301390
Emerg Infect Dis. 2020 Jun;26(6):1266-1273
pubmed: 32160149
Euro Surveill. 2020 Apr;25(13):
pubmed: 32265007
J Virol. 2020 Jul 1;94(14):
pubmed: 32357959
Ann Intern Med. 2020 Aug 4;173(3):242-243
pubmed: 32302380
N Engl J Med. 2020 Mar 26;382(13):1278-1280
pubmed: 32069388
Indian J Med Res. 2020 Feb & Mar;151(2 & 3):255-260
pubmed: 32362650
Nature. 2020 Mar;579(7798):265-269
pubmed: 32015508
Indian J Med Res. 2020 Feb & Mar;151(2 & 3):200-209
pubmed: 32242873
J Virol. 2020 May 18;94(11):
pubmed: 32238585
Lancet. 2020 Feb 15;395(10223):514-523
pubmed: 31986261
Osong Public Health Res Perspect. 2020 Feb;11(1):3-7
pubmed: 32149036
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
PLoS One. 2018 Feb 22;13(2):e0192672
pubmed: 29470509
Emerg Infect Dis. 2020 Jul;26(7):1542-1547
pubmed: 32315281
Natl Sci Rev. 2020 Jun;7(6):1012-1023
pubmed: 34676127
Nat Microbiol. 2020 Apr;5(4):536-544
pubmed: 32123347
J Transl Med. 2020 Apr 22;18(1):179
pubmed: 32321524
J Virol. 2020 Aug 17;94(17):
pubmed: 32571797
Clin Microbiol Infect. 2020 Jul;26(7):954-956
pubmed: 32229288