Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging.


Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
23 03 2021
Historique:
pubmed: 10 3 2021
medline: 22 6 2021
entrez: 9 3 2021
Statut: ppublish

Résumé

Despite being a critical molecule in the brain, mass spectrometry imaging (MSI) of cholesterol has been under-reported compared to other lipids due to the difficulty in ionizing the sterol molecule. In the present work, we have employed an on-tissue enzyme-assisted derivatization strategy to improve detection of cholesterol in brain tissue sections. We report distribution and levels of cholesterol across specific structures of the mouse brain, in a model of Niemann-Pick type C1 disease, and during brain development. MSI revealed that in the adult mouse, cholesterol is the highest in the pons and medulla and how its distribution changes during development. Cholesterol was significantly reduced in the corpus callosum and other brain regions in the

Identifiants

pubmed: 33687199
doi: 10.1021/acs.analchem.0c05399
pmc: PMC7992047
doi:

Substances chimiques

Sterols 0
Cholesterol 97C5T2UQ7J

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4932-4943

Subventions

Organisme : NIMH NIH HHS
ID : R01 MH110636
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N015932/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L001942/1
Pays : United Kingdom

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Auteurs

Roberto Angelini (R)

Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.

Eylan Yutuc (E)

Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.

Mark F Wyatt (MF)

Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.

Jillian Newton (J)

Centre for Mass Spectrometry Imaging, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, U.K.

Fowzi A Yusuf (FA)

Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.

Lauren Griffiths (L)

Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.

Benjamin J Cooze (BJ)

Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.

Dana El Assad (D)

Materials Research and Technology, Luxembourg Institute of Science and Technology, Belvaux L-4422, Luxembourg.

Gilles Frache (G)

Materials Research and Technology, Luxembourg Institute of Science and Technology, Belvaux L-4422, Luxembourg.

Wei Rao (W)

European Application Laboratory, Waters Corporation, Stamford Avenue, Altrincham Road, Wilmslow SK9 4AX, U.K.

Luke B Allen (LB)

Departments of Pediatrics and Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198, United States.

Zeljka Korade (Z)

Departments of Pediatrics and Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198, United States.

Thu T A Nguyen (TTA)

Department of Chemistry and Laboratory of Integrated Neuroscience, University of Illinois at Chicago, Chicago, Illinois 60607, United States.

Rathnayake A C Rathnayake (RAC)

Department of Chemistry and Laboratory of Integrated Neuroscience, University of Illinois at Chicago, Chicago, Illinois 60607, United States.

Stephanie M Cologna (SM)

Department of Chemistry and Laboratory of Integrated Neuroscience, University of Illinois at Chicago, Chicago, Illinois 60607, United States.

Owain W Howell (OW)

Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.

Malcolm R Clench (MR)

Centre for Mass Spectrometry Imaging, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, U.K.

Yuqin Wang (Y)

Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.

William J Griffiths (WJ)

Medical School, Swansea University, Singleton Park, Swansea SA2 8PP, Wales, U.K.

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Classifications MeSH