UGT84F9 is the major flavonoid UDP-glucuronosyltransferase in Medicago truncatula.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
23 04 2021
23 04 2021
Historique:
received:
28
07
2020
accepted:
07
01
2021
pubmed:
12
3
2021
medline:
31
8
2021
entrez:
11
3
2021
Statut:
ppublish
Résumé
Mammalian phase II metabolism of dietary plant flavonoid compounds generally involves substitution with glucuronic acid. In contrast, flavonoids mainly exist as glucose conjugates in plants, and few plant UDP-glucuronosyltransferase enzymes have been identified to date. In the model legume Medicago truncatula, the major flavonoid compounds in the aerial parts of the plant are glucuronides of the flavones apigenin and luteolin. Here we show that the M. truncatula glycosyltransferase UGT84F9 is a bi-functional glucosyl/glucuronosyl transferase in vitro, with activity against a wide range of flavonoid acceptor molecules including flavones. However, analysis of metabolite profiles in leaves and roots of M. truncatula ugt84f9 loss of function mutants revealed that the enzyme is essential for formation of flavonoid glucuronides, but not most flavonoid glucosides, in planta. We discuss the use of plant UGATs for the semi-synthesis of flavonoid phase II metabolites for clinical studies.
Identifiants
pubmed: 33694362
pii: 6122440
doi: 10.1093/plphys/kiab016
pmc: PMC8133618
doi:
Substances chimiques
Flavonoids
0
Glucuronosyltransferase
EC 2.4.1.17
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1617-1637Subventions
Organisme : NCCIH NIH HHS
ID : P50 AT008661
Pays : United States
Informations de copyright
© American Society of Plant Biologists 2021. All rights reserved. For permissions, please email: journals.permissions@oup.com.
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