Mitochondrial DNA Analysis from Exome Sequencing Data Improves Diagnostic Yield in Neurological Diseases.


Journal

Annals of neurology
ISSN: 1531-8249
Titre abrégé: Ann Neurol
Pays: United States
ID NLM: 7707449

Informations de publication

Date de publication:
06 2021
Historique:
revised: 05 03 2021
received: 06 11 2020
accepted: 08 03 2021
pubmed: 12 3 2021
medline: 3 8 2021
entrez: 11 3 2021
Statut: ppublish

Résumé

A rapidly expanding catalog of neurogenetic disorders has encouraged a diagnostic shift towards early clinical whole exome sequencing (WES). Adult primary mitochondrial diseases (PMDs) frequently exhibit neurological manifestations that overlap with other nervous system disorders. However, mitochondrial DNA (mtDNA) is not routinely analyzed in standard clinical WES bioinformatic pipelines. We reanalyzed 11,424 exomes, enriched with neurological diseases, for pathogenic mtDNA variants. Twenty-four different mtDNA mutations were detected in 64 exomes, 11 of which were considered disease causing based on the associated clinical phenotypes. These findings highlight the diagnostic uplifts gained by analyzing mtDNA from WES data in neurological diseases. ANN NEUROL 2021;89:1240-1247.

Identifiants

pubmed: 33704825
doi: 10.1002/ana.26063
pmc: PMC8494076
doi:

Substances chimiques

DNA, Mitochondrial 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1240-1247

Subventions

Organisme : Wellcome Trust
ID : Wellcome Trust Synaptopathies Award
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/J004758/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S002065/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S005021/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0802760
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1001253
Pays : United Kingdom

Investigateurs

Issam Alkhawaja (I)
Selina Banu (S)
Maria Bonsignore (M)
Marianthi Breza (M)
Gabriella Di Rosa (G)
Morteza Heidari (M)
Georgios Koutsis (G)
Arn M J M van den Maagdenberg (AMJM)
Alfons Macaya (A)
Alexander Münchau (A)
Carmela Scuderi (C)
Nazira Zharkinbekova (N)

Informations de copyright

© 2021 The Authors. Annals of Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association.

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Auteurs

Olivia V Poole (OV)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Chiara Pizzamiglio (C)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

David Murphy (D)

Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Micol Falabella (M)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology, London, UK.

William L Macken (WL)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Enrico Bugiardini (E)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Cathy E Woodward (CE)

Neurogenetics Unit, The National Hospital for Neurology and Neurosurgery, London, UK.

Robyn Labrum (R)

Neurogenetics Unit, The National Hospital for Neurology and Neurosurgery, London, UK.

Stephanie Efthymiou (S)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Vincenzo Salpietro (V)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Viorica Chelban (V)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Rauan Kaiyrzhanov (R)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Reza Maroofian (R)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Anthony A Amato (AA)

Department of Neurology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.

Allison Gregory (A)

Departments of Molecular and Medical Genetics, Pediatrics, and Neurology, Oregon Health and Science University, Portland, OR.

Susan J Hayflick (SJ)

Departments of Molecular and Medical Genetics, Pediatrics, and Neurology, Oregon Health and Science University, Portland, OR.
UCL Queen Square Institute of Neurology, London, UK.

Hallgeir Jonvik (H)

Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Nicholas Wood (N)

Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Henry Houlden (H)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Jana Vandrovcova (J)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Michael G Hanna (MG)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

Alan Pittman (A)

Genetics Research Centre, St. George's, University of London, London, UK.

Robert D S Pitceathly (RDS)

Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology and The National Hospital for Neurology and Neurosurgery, London, UK.

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