Electrochemiluminescence imaging of respiratory activity of cellular spheroids using sequential potential steps.
Electrochemiluminescence
Live-cell imaging
Luminol
Mesenchymal stem cell spheroid
Oxygen consumption
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
01 Jun 2021
01 Jun 2021
Historique:
received:
21
12
2020
revised:
10
02
2021
accepted:
24
02
2021
pubmed:
15
3
2021
medline:
15
5
2021
entrez:
14
3
2021
Statut:
ppublish
Résumé
The respiratory activity of cultured cells can be electrochemically monitored using scanning electrochemical microscopy (SECM) with high spatial resolution. However, in SECM, the electrode takes a long time to scan, limiting simultaneous measurements with large biological samples such as cell spheroids. Therefore, for rapid electrochemical imaging, a novel strategy is needed. Herein, we report electrochemiluminescence (ECL) imaging of spheroid respiratory activity for the first time using sequential potential steps. L-012, a luminol analog, was used as an ECL luminophore, and H
Identifiants
pubmed: 33714859
pii: S0956-5663(21)00160-3
doi: 10.1016/j.bios.2021.113123
pii:
doi:
Substances chimiques
Luminol
5EXP385Q4F
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
113123Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.