Repeated binge ethanol drinking enhances electrical activity of central amygdala corticotropin releasing factor neurons in vivo.
Binge ethanol drinking
CRF
CeA
Electrophysiology
Ethanol
Journal
Neuropharmacology
ISSN: 1873-7064
Titre abrégé: Neuropharmacology
Pays: England
ID NLM: 0236217
Informations de publication
Date de publication:
15 05 2021
15 05 2021
Historique:
received:
11
11
2020
revised:
11
02
2021
accepted:
05
03
2021
pubmed:
21
3
2021
medline:
15
12
2021
entrez:
20
3
2021
Statut:
ppublish
Résumé
Binge ethanol drinking is an increasingly problematic component of alcohol use disorder costing the United States approximately over $150 billion every year and causes progressive neuroplasticity alterations in numerous brain regions. However, the precise nature or machinery that underlies binge drinking has not yet been elucidated. Corticotropin releasing factor (CRF) neurons in the central amygdala (CeA) are thought to modulate binge drinking, but the specific circuit mechanisms remain poorly understood. Here, we combined optogenetics with in vivo electrophysiology to identify and record from CeA CRF neurons in mice during a repeated binge ethanol drinking task. First, we found that CeA CRF neurons were more active than CeA non-CRF cells during our binge drinking paradigm. We also observed that CeA CRF neurons displayed a heterogeneous spectrum of responses to a lick of ethanol including, pre-lick activated, lick-excited, lick-inhibited, and no response. Interestingly, pre-lick activated CeA CRF neurons exhibited higher frequency and burst firing during binge drinking sessions. Moreover, their overall tonic and phasic electrical activity enhances over repeated binge drinking sessions. Remarkably, CeA CRF units and pre-lick activated CeA CRF neurons did not show higher firing rate or bursting activity during water and sucrose consumption, suggesting that ethanol may "hijack" or plastically alter their intrinsic excitability. This article is part of the special issue on 'Neurocircuitry Modulating Drug and Alcohol Abuse'.
Identifiants
pubmed: 33741403
pii: S0028-3908(21)00081-2
doi: 10.1016/j.neuropharm.2021.108527
pmc: PMC8928451
mid: NIHMS1780919
pii:
doi:
Substances chimiques
Ethanol
3K9958V90M
Corticotropin-Releasing Hormone
9015-71-8
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
108527Subventions
Organisme : NIDA NIH HHS
ID : R01 DA045639
Pays : United States
Organisme : NIAAA NIH HHS
ID : R01 AA027516
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA022340
Pays : United States
Organisme : NIAAA NIH HHS
ID : R00 AA021417
Pays : United States
Organisme : NIDA NIH HHS
ID : F31 DA047050
Pays : United States
Informations de copyright
Copyright © 2021. Published by Elsevier Ltd.
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