Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin.
C1C2
Chlamydomonas reinhardtii
channelrhodopsin
chlamydomonas reinhardtii
molecular biophysics
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
23 03 2021
23 03 2021
Historique:
received:
23
08
2020
accepted:
15
02
2021
entrez:
23
3
2021
pubmed:
24
3
2021
medline:
4
9
2021
Statut:
epublish
Résumé
Channelrhodopsins (ChRs) are microbial light-gated ion channels utilized in optogenetics to control neural activity with light . Light absorption causes retinal chromophore isomerization and subsequent protein conformational changes visualized as optically distinguished intermediates, coupled with channel opening and closing. However, the detailed molecular events underlying channel gating remain unknown. We performed time-resolved serial femtosecond crystallographic analyses of ChR by using an X-ray free electron laser, which revealed conformational changes following photoactivation. The isomerized retinal adopts a twisted conformation and shifts toward the putative internal proton donor residues, consequently inducing an outward shift of TM3, as well as a local deformation in TM7. These early conformational changes in the pore-forming helices should be the triggers that lead to opening of the ion conducting pore.
Identifiants
pubmed: 33752801
doi: 10.7554/eLife.62389
pii: 62389
pmc: PMC7987342
doi:
pii:
Substances chimiques
Algal Proteins
0
Channelrhodopsins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 16H06294
Organisme : Japan Society for the Promotion of Science
ID : 18J21256
Organisme : Japan Society for the Promotion of Science
ID : 17H05000
Organisme : Japan Science and Technology Agency
ID : JPMJPR14L9
Organisme : Japan Science and Technology Agency
ID : JPMJPR14L8
Organisme : Japan Agency for Medical Research and Development
ID : Platform Project for Supporting Drug Discovery andLife Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research(BINDS))
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB1078 (B2)
Organisme : Cluster of Excellence in Inflammation Research
ID : UniCat
Organisme : Cluster of Excellence in Inflammation Research
ID : BIG-NSE
Organisme : Cluster of Excellence in Inflammation Research
ID : E4
Organisme : Cluster of Excellence in Inflammation Research
ID : SPP 1926
Organisme : European Research Council
ID : ERC-2016-StG 714762
Pays : International
Organisme : Hertie Foundation
ID : Senior Research Professor
Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : X-ray Free-Electron Laser Priority Strategy Program
Informations de copyright
© 2021, Oda et al.
Déclaration de conflit d'intérêts
KO, TN, TN, KY, KI, SI, JV, KH, AM, KK, TI, II, TI, RU, RE, SO, GK, TK, TK, WS, HS, TT, MT, RT, AT, RN, MF, HM, YL, EN, RT, TT, MS, TK, TS, TF, YY, SO, YJ, KT, RI, SH, HK, PH, SI, MK, TN, ON No competing interests declared
Références
J Appl Crystallogr. 2016 Apr 18;49(Pt 3):1035-1041
pubmed: 27275146
Science. 2018 Jul 13;361(6398):
pubmed: 29903883
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2184-95
pubmed: 15572771
Annu Rev Biophys. 2015;44:167-86
pubmed: 26098512
Angew Chem Int Ed Engl. 2015 Apr 13;54(16):4953-7
pubmed: 25537168
Cell. 2011 Dec 23;147(7):1446-57
pubmed: 22196724
Nature. 2017 Mar 2;543(7643):131-135
pubmed: 28219079
Biochemistry. 2019 Mar 5;58(9):1275-1286
pubmed: 30702875
Biochemistry. 2010 Jan 19;49(2):267-78
pubmed: 20000562
Nature. 2012 Jan 22;482(7385):369-74
pubmed: 22266941
Rev Sci Instrum. 2014 Mar;85(3):033110
pubmed: 24689567
Chemphyschem. 2010 Apr 26;11(6):1120-6
pubmed: 20349494
J Mol Biol. 2011 Nov 18;414(1):86-95
pubmed: 22001017
Proc Natl Acad Sci U S A. 2015 Oct 27;112(43):E5796-804
pubmed: 26460012
Sci Rep. 2017 Aug 3;7(1):7217
pubmed: 28775289
Nat Commun. 2013;4:1678
pubmed: 23575682
J Biol Chem. 2008 Dec 12;283(50):35033-41
pubmed: 18927082
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
Nature. 2020 Jul;583(7815):314-318
pubmed: 32499654
Nat Commun. 2017 Nov 17;8(1):1585
pubmed: 29147002
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13940-5
pubmed: 14615590
J Biol Chem. 2012 Mar 2;287(10):7456-66
pubmed: 22241469
Sci Adv. 2017 Jul 14;3(7):e1603042
pubmed: 28740863
Nat Neurosci. 2009 Feb;12(2):229-34
pubmed: 19079251
J Am Chem Soc. 2014 Mar 5;136(9):3475-82
pubmed: 24512107
J Phys Chem Lett. 2018 Dec 20;9(24):7180-7184
pubmed: 30525663
Proc Natl Acad Sci U S A. 2019 May 7;116(19):9380-9389
pubmed: 31004059
Photochem Photobiol Sci. 2010 Feb;9(2):194-8
pubmed: 20126794
Elife. 2021 Mar 23;10:
pubmed: 33752801
J Biol Chem. 2015 May 1;290(18):11623-34
pubmed: 25796616
Chem Rev. 2014 Jan 8;114(1):126-63
pubmed: 24364740
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Nat Commun. 2016 Nov 17;7:13415
pubmed: 27853152
Biophys J. 2018 Oct 2;115(7):1281-1291
pubmed: 30236783
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):E1273-81
pubmed: 23509282
J Biochem Biophys Methods. 1998 Jun 11;36(2-3):157-73
pubmed: 9711501
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Nat Commun. 2018 Sep 26;9(1):3949
pubmed: 30258177
Biochim Biophys Acta. 2002 Oct 11;1565(2):144-67
pubmed: 12409192
Science. 2016 Dec 23;354(6319):1552-1557
pubmed: 28008064
J Mol Biol. 2008 Jan 18;375(3):686-94
pubmed: 18037436
J Am Chem Soc. 2013 May 8;135(18):6968-76
pubmed: 23537405
Nature. 2011 Jul 27;477(7363):171-8
pubmed: 21796121
J Appl Crystallogr. 2014 May 29;47(Pt 3):1118-1131
pubmed: 24904246
J Mol Biol. 2015 Jan 30;427(2):341-9
pubmed: 25451024