Epidemiology of Chikungunya virus isolates 2016-2018 in Pakistan.
Adolescent
Adult
Aged
Amino Acid Substitution
Chikungunya Fever
/ epidemiology
Chikungunya virus
/ genetics
Child
Child, Preschool
Disease Outbreaks
Female
Genome, Viral
Humans
Infant
Male
Middle Aged
Mutation, Missense
Pakistan
/ epidemiology
Phylogeny
Sequence Analysis, DNA
Sequence Homology
Serum
/ virology
Young Adult
Chikungunia virus
endemic infection
epidemiology
mutation
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
22
02
2021
received:
31
12
2020
accepted:
04
03
2021
pubmed:
24
3
2021
medline:
8
2
2022
entrez:
23
3
2021
Statut:
ppublish
Résumé
The chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus, which has infected millions of people in Africa, Asia, Americas, and Europe since it remerged in India and Indian Ocean regions in 2005-2006. The purpose of this study was to evaluate the genetic diversity and evolutionary changes in CHIKV from 2016 to 2018 in Pakistan. Blood specimens were collected and processed following the Centers for Disease Control and Prevention Trioplex Protocol. Sequencing and phylogenetic analysis of complete coding sequence of representative isolates from the CHIKV outbreak was carried out during December 2016 to July 2018, a total of 1549 samples were received, out of which 50% (n = 774) were found positive for CHIKV RNA. Mean age of chikungunya positive patients was 31.8 ± 15.7 years and most affected were between 21 and 40 years of age. The Pakistan CHIKV strains clustered with the Indian Ocean sublineage of East/Central/South African with cocirculation of some variants In the structural proteins region, two noteworthy changes (A226V and D284E) were observed in the membrane fusion glycoprotein E1. Key substitutions in the neutralizing epitopes site and a few changes indicative of adaptive to other insect cells were also detected in Pakistani strains. This study provides the emerging trend of CHIKV in the country for early identification of potential variants of high virulence and preventive measures for vector borne disease especially in the endemic areas.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6124-6131Informations de copyright
© 2021 Wiley Periodicals LLC.
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