Gene expression patterns of Cydia pomonella granulovirus in codling moth larvae revealed by RNAseq analysis.

Animals Gene Expression Profiling Genes, Viral Granulovirus / genetics Larva / virology Moths / virology Sequence Analysis, RNA / methods Transcriptome Virus Replication
Baculoviridae Betabaculovirus CpGV Cydia pomonella In vivo infection RNA isolation Time course Transcriptome Virus replication

Journal

Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674

Informations de publication

Date de publication:
06 2021
Historique:
received: 24 11 2020
revised: 13 02 2021
accepted: 15 02 2021
pubmed: 24 3 2021
medline: 29 12 2021
entrez: 23 3 2021
Statut: ppublish

Résumé

The Cydia pomonella granulovirus (CpGV) has been used as a biological control agent of codling moth (Cydia pomonella), a severe global pest on pome fruit. Despite the economic importance, our knowledge of its molecular biology is still limited and a detailed picture of its gene expression is still missing. Here, we sequenced the transcriptome of codling moth larvae infected with the Mexican isolate CpGV-M and analyzed the expression of viral genes at 12, 48, and 96 h post infection (hpi). The results showed that two genes (p6.9 and pp31/39K) related to DNA binding of virus production, were highly expressed at 48 and 96 hpi. From 48 to 96 hpi, the expression of genes associated with virus replication and dissemination decreased, whereas the expression of genes related to infectious virion production and per os infectivity increased. This study provides a comprehensive view of CpGV gene expression patterns in host larvae.

Identifiants

DOI: 10.1016/j.virol.2021.02.015 PMID: 33756423
pubmed: 33756423
pii: S0042-6822(21)00055-6
doi: 10.1016/j.virol.2021.02.015
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

110-118

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Yu Xi (Y)

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China; Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Biological Control, Heinrichstraße 243, 64287, Darmstadt, Germany.

Longsheng Xing (L)

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.

Jörg T Wennmann (JT)

Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Biological Control, Heinrichstraße 243, 64287, Darmstadt, Germany.

Jiangbin Fan (J)

Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Biological Control, Heinrichstraße 243, 64287, Darmstadt, Germany.

Zaiyuan Li (Z)

College of Agriculture, Yangtze University, Jingzhou, 434025, China.

Qiang Wu (Q)

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.

Sha Lu (S)

College of Plant Health & Medicine, Qingdao Agricultural University, Qingdao, 266109, China.

Bo Liu (B)

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.

Jianyang Guo (J)

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Xi Qiao (X)

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.

Cong Huang (C)

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.

Wanqiang Qian (W)

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China. Electronic address: qianwanqiang@caas.cn.

Johannes A Jehle (JA)

Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Biological Control, Heinrichstraße 243, 64287, Darmstadt, Germany. Electronic address: johannes.jehle@julius-kuehn.de.

Fanghao Wan (F)

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China; College of Plant Health & Medicine, Qingdao Agricultural University, Qingdao, 266109, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: wanfanghao@caas.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Gene expression patterns of Cydia pomonella...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de profil d'expression de gènes › Gene expression patterns of Cydia pomonella...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome viral › Gènes viraux › Gene expression patterns of Cydia pomonella...
questionsmedicales.fr › Virus › Virus des insectes › Baculoviridae › Granulovirus › Gene expression patterns of Cydia pomonella...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Métamorphose biologique › Étapes du cycle de vie › Larve › Gene expression patterns of Cydia pomonella...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Lepidoptera › Papillons de nuit › Gene expression patterns of Cydia pomonella...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ARN › Gene expression patterns of Cydia pomonella...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Transcriptome › Gene expression patterns of Cydia pomonella...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Gene expression patterns of Cydia pomonella...