Profiling the Gastrointestinal Microbiota.

Bacteria / classification DNA, Bacterial / genetics DNA, Ribosomal / genetics Feces / microbiology Gastrointestinal Microbiome High-Throughput Nucleotide Sequencing Humans Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA / methods
16S ribosomal DNA Microbial community Next-generation sequencing PCR amplification Sequence-based analysis Stool samples Targeted amplicon sequencing

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2021
Historique:
entrez: 25 3 2021
pubmed: 26 3 2021
medline: 12 6 2021
Statut: ppublish

Résumé

In this chapter, we provide a methodological description of the process to perform gastrointestinal (GIT) microbiota profiling on human stool samples. The process includes: (i) collection of feces, (ii) isolation of DNA from fecal community bacteria, (iii) selection of both 16S rDNA sequencing target and next-generation sequencing platform, and (iv) analysis and interpretation of sequence data. The process culminates into a comprehensive report on the GIT microbiota composition and structure that may translate into clinically actionable results.

Identifiants

DOI: 10.1007/978-1-0716-1302-3_10 PMID: 33765312
pubmed: 33765312
doi: 10.1007/978-1-0716-1302-3_10
doi:

Substances chimiques

DNA, Bacterial 0
DNA, Ribosomal 0
RNA, Ribosomal, 16S 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

83-92

Références

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Auteurs

Brunella Posteraro (B)

Dipartimento di Scienze Mediche e Chirurgiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy. brunella.posteraro@unicatt.it.

Flavio De Maio (F)

Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.

Antonio Gasbarrini (A)

Dipartimento di Scienze Mediche e Chirurgiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Profiling the Gastrointestinal Microbiota.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN bactérien › Profiling the Gastrointestinal Microbiota.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN ribosomique › Profiling the Gastrointestinal Microbiota.
questionsmedicales.fr › Liquides et sécrétions biologiques › Fèces › Profiling the Gastrointestinal Microbiota.
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbiome gastro-intestinal › Profiling the Gastrointestinal Microbiota.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Séquençage nucléotidique à haut débit › Profiling the Gastrointestinal Microbiota.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Profiling the Gastrointestinal Microbiota.
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Profiling the Gastrointestinal Microbiota.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Profiling the Gastrointestinal Microbiota.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Profiling the Gastrointestinal Microbiota.