Identification and localization of Nup170 in the microsporidian Nosema bombycis.
Immunofluorescence
Nosema bombycis
Nuclear pore complex
Nucleoporins
RNA interference
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
05
10
2020
accepted:
17
03
2021
pubmed:
27
3
2021
medline:
23
6
2021
entrez:
26
3
2021
Statut:
ppublish
Résumé
As one of the core framework proteins of nuclear pore complex (NPC), nucleoporin Nupl70 acts as a structural adapter between the nucleolus and nuclear pore membrane and maintains the stability of NPC structure through interaction with other proteins. In this study, we identified a Nup170 protein in the microsporidian Nosema bombycis for the first time and named it as NbNup170. Secondary structure prediction showed that the NbNup170 contains α-helices and random coils. The three-dimensional structure of NbNup170 is elliptical in shape. Phylogenetic analysis based on the Nup170 and homologous sequences showed that N. bombycis clustered together with Vairimorpha ceranae and Vairimorpha apis. The immunofluorescence localization results showed that the NbNup170 was located on the plasma membrane of the dormant spore and transferred to the surface of sporoplasm in a punctate pattern when the dormant spore has finished germination, and that NbNup170 was distributed on the nuclear membrane and both sides of the nuclei of early proliferative phase, and only on the nuclear membrane during sporogonic phase in the N. bombycis. qPCR analysis showed that the relative expression level of NbNup170 maintained at a low level from 30 to 78 h post-infection with N. bombycis, then reached the highest at 102 h, while that of NbNup170 was repressed at a very low level throughout its life cycle by RNA interference. These results suggested that NbNup170 protein is involved in the proliferative phase and active during the sporogonic phase of N. bombycis.
Identifiants
pubmed: 33768334
doi: 10.1007/s00436-021-07129-4
pii: 10.1007/s00436-021-07129-4
doi:
Substances chimiques
Fungal Proteins
0
Nuclear Pore Complex Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2125-2134Subventions
Organisme : National Natural Science Foundation of China
ID : 31960684
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