Synergistic Effect of Barbadensis miller and Marsdenia Condurango Extracts Induces Apoptosis Promotes Oxidative Stress by Limiting Proliferation of Cervical Cancer and Liver Cancer Cells.
Aloe
Apoptosis
/ drug effects
Carcinoma, Hepatocellular
Catalase
/ drug effects
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Drug Synergism
Female
Glutathione
/ drug effects
HeLa Cells
Hep G2 Cells
Humans
Inhibitory Concentration 50
Liver Neoplasms
Marsdenia
Oxidative Stress
/ drug effects
Phytotherapy
Plant Extracts
/ pharmacology
Superoxide Dismutase
/ drug effects
Tumor Suppressor Protein p53
/ drug effects
Uterine Cervical Neoplasms
Cytotoxicity
Gene expression
Protein expression
Synergistic effect
Journal
Asian Pacific journal of cancer prevention : APJCP
ISSN: 2476-762X
Titre abrégé: Asian Pac J Cancer Prev
Pays: Thailand
ID NLM: 101130625
Informations de publication
Date de publication:
01 Mar 2021
01 Mar 2021
Historique:
received:
04
11
2020
entrez:
28
3
2021
pubmed:
29
3
2021
medline:
17
11
2021
Statut:
epublish
Résumé
Drug synergy is the combine effect of drug efficacy. Synergistic combinations of active ingredients have proven to be highly effective and more useful in therapeutics. In contrast, the individual effect of drug is usually undesirable and mostly used for selecting drug-resistant mutations. Purpose of this study was to check synergistic effects of both plants (Barbadensis miller and Marsdenia condurango) against liver and cervical cancer. Culturing of HeLa (cervical cancer cell line) and HepG2 (liver cancer cell line) cells, IC50 evaluation, viability assays (trypan blue, crystal violet), p53 ELISA and immunocytochemistry, MUSE analysis (count and viability), antioxidants (GSH, SOD, CAT), at the end RT-PCR was performed. IC50 evaluation was done of each plant individually and with combination for synergistic effects, IC50 with plants combination (synergism) was applied on further viability assays (trypan blue, crystal violet, MUSE analysis via count and viability kit) p53 ELISA and immunocytochemistry for evaluation of cellular apoptosis, antioxidants assays (GSH, SOD, CAT), and RT-PCR with proliferative and apoptotic markers along with internal control. According to current study it was observed that synergistic effect of these plants has more anticancer properties with minimum effective dose. It was also observed that extracts possess the ability to induce apoptosis, restrict proliferation and enhanced oxidative stress.
Sections du résumé
BACKGROUND
BACKGROUND
Drug synergy is the combine effect of drug efficacy. Synergistic combinations of active ingredients have proven to be highly effective and more useful in therapeutics. In contrast, the individual effect of drug is usually undesirable and mostly used for selecting drug-resistant mutations. Purpose of this study was to check synergistic effects of both plants (Barbadensis miller and Marsdenia condurango) against liver and cervical cancer.
METHODOLOGY
METHODS
Culturing of HeLa (cervical cancer cell line) and HepG2 (liver cancer cell line) cells, IC50 evaluation, viability assays (trypan blue, crystal violet), p53 ELISA and immunocytochemistry, MUSE analysis (count and viability), antioxidants (GSH, SOD, CAT), at the end RT-PCR was performed.
RESULTS
RESULTS
IC50 evaluation was done of each plant individually and with combination for synergistic effects, IC50 with plants combination (synergism) was applied on further viability assays (trypan blue, crystal violet, MUSE analysis via count and viability kit) p53 ELISA and immunocytochemistry for evaluation of cellular apoptosis, antioxidants assays (GSH, SOD, CAT), and RT-PCR with proliferative and apoptotic markers along with internal control.
CONCLUSION
CONCLUSIONS
According to current study it was observed that synergistic effect of these plants has more anticancer properties with minimum effective dose. It was also observed that extracts possess the ability to induce apoptosis, restrict proliferation and enhanced oxidative stress.
Identifiants
pubmed: 33773549
doi: 10.31557/APJCP.2021.22.3.843
pmc: PMC8286677
pii:
doi:
Substances chimiques
Plant Extracts
0
TP53 protein, human
0
Tumor Suppressor Protein p53
0
Catalase
EC 1.11.1.6
Superoxide Dismutase
EC 1.15.1.1
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
843-852Références
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