Nascent Prostate Cancer Heterogeneity Drives Evolution and Resistance to Intense Hormonal Therapy.

Androgen deprivation therapy Diversity Enzalutamide Evolution Genomics Immunohistochemistry Neoadjuvant Prostate cancer

Journal

European urology
ISSN: 1873-7560
Titre abrégé: Eur Urol
Pays: Switzerland
ID NLM: 7512719

Informations de publication

Date de publication:
Dec 2021
Historique:
received: 09 10 2020
accepted: 11 03 2021
pubmed: 1 4 2021
medline: 19 4 2022
entrez: 31 3 2021
Statut: ppublish

Résumé

Patients diagnosed with high risk localized prostate cancer have variable outcomes following surgery. Trials of intense neoadjuvant androgen deprivation therapy (NADT) have shown lower rates of recurrence among patients with minimal residual disease after treatment. The molecular features that distinguish exceptional responders from poor responders are not known. To identify genomic and histologic features associated with treatment resistance at baseline. Targeted biopsies were obtained from 37 men with intermediate- to high-risk prostate cancer before receiving 6 mo of ADT plus enzalutamide. Biopsy tissues were used for whole-exome sequencing and immunohistochemistry (IHC). We assessed the relationship of molecular features with final pathologic response using a cutpoint of 0.05 cm Loss of chromosome 10q (containing PTEN) and alterations to TP53 were predictive of poor response, as were the expression of nuclear ERG on IHC and the presence of intraductal carcinoma of the prostate. Patients with incompletely and nonresponding tumors harbored greater tumor diversity as estimated via phylogenetic tree reconstruction from DNA sequencing and analysis of IHC staining. Our four-factor binary model (area under the receiver operating characteristic curve [AUC] 0.89) to predict poor response correlated with greater diversity in our cohort and a validation cohort of 57 Gleason score 8-10 prostate cancers from The Cancer Genome Atlas. When baseline tumor volume was added to the model, it distinguished poor response to NADT with an AUC of 0.98. Prospective use of this model requires further retrospective validation with biopsies from additional trials. A subset of prostate cancers exhibit greater histologic and genomic diversity at the time of diagnosis, and these localized tumors have greater fitness to resist therapy. Some prostate cancer tumors do not respond well to a hormonal treatment called androgen deprivation therapy (ADT). We used tumor volume and four other parameters to develop a model to identify tumors that will not respond well to ADT. Treatments other than ADT should be considered for these patients.

Sections du résumé

BACKGROUND BACKGROUND
Patients diagnosed with high risk localized prostate cancer have variable outcomes following surgery. Trials of intense neoadjuvant androgen deprivation therapy (NADT) have shown lower rates of recurrence among patients with minimal residual disease after treatment. The molecular features that distinguish exceptional responders from poor responders are not known.
OBJECTIVE OBJECTIVE
To identify genomic and histologic features associated with treatment resistance at baseline.
DESIGN, SETTING, AND PARTICIPANTS METHODS
Targeted biopsies were obtained from 37 men with intermediate- to high-risk prostate cancer before receiving 6 mo of ADT plus enzalutamide. Biopsy tissues were used for whole-exome sequencing and immunohistochemistry (IHC).
OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS METHODS
We assessed the relationship of molecular features with final pathologic response using a cutpoint of 0.05 cm
RESULTS AND LIMITATIONS CONCLUSIONS
Loss of chromosome 10q (containing PTEN) and alterations to TP53 were predictive of poor response, as were the expression of nuclear ERG on IHC and the presence of intraductal carcinoma of the prostate. Patients with incompletely and nonresponding tumors harbored greater tumor diversity as estimated via phylogenetic tree reconstruction from DNA sequencing and analysis of IHC staining. Our four-factor binary model (area under the receiver operating characteristic curve [AUC] 0.89) to predict poor response correlated with greater diversity in our cohort and a validation cohort of 57 Gleason score 8-10 prostate cancers from The Cancer Genome Atlas. When baseline tumor volume was added to the model, it distinguished poor response to NADT with an AUC of 0.98. Prospective use of this model requires further retrospective validation with biopsies from additional trials.
CONCLUSIONS CONCLUSIONS
A subset of prostate cancers exhibit greater histologic and genomic diversity at the time of diagnosis, and these localized tumors have greater fitness to resist therapy.
PATIENT SUMMARY RESULTS
Some prostate cancer tumors do not respond well to a hormonal treatment called androgen deprivation therapy (ADT). We used tumor volume and four other parameters to develop a model to identify tumors that will not respond well to ADT. Treatments other than ADT should be considered for these patients.

Identifiants

pubmed: 33785256
pii: S0302-2838(21)00207-4
doi: 10.1016/j.eururo.2021.03.009
pmc: PMC8473585
mid: NIHMS1684972
pii:
doi:

Substances chimiques

Androgen Antagonists 0
Androgens 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

746-757

Subventions

Organisme : Intramural NIH HHS
ID : ZIA BC010666
Pays : United States
Organisme : CCR NIH HHS
ID : HHSN261200800001C
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA BC011838
Pays : United States
Organisme : Intramural NIH HHS
ID : Z99 CA999999
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261200800001E
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA BC011679
Pays : United States

Commentaires et corrections

Type : CommentIn
Type : CommentIn

Informations de copyright

Published by Elsevier B.V.

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Auteurs

Scott Wilkinson (S)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA.

Huihui Ye (H)

Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA; Department of Pathology and Department of Urology, University of California-Los Angeles, Los Angeles, CA, USA.

Fatima Karzai (F)

Genitourinary Malignancies Branch, National Cancer Institute, Bethesda, MD, USA.

Stephanie A Harmon (SA)

Molecular Imaging Branch, National Cancer Institute, Bethesda, MD, USA; Clinical Research Directorate, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.

Nicholas T Terrigino (NT)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA.

David J VanderWeele (DJ)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA; Department of Medicine, Feinberg School of Medicine, Chicago, IL, USA.

John R Bright (JR)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA.

Rayann Atway (R)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA.

Shana Y Trostel (SY)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA.

Nicole V Carrabba (NV)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA.

Nichelle C Whitlock (NC)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA.

Stephanie M Walker (SM)

Molecular Imaging Branch, National Cancer Institute, Bethesda, MD, USA.

Rosina T Lis (RT)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA.

Houssein Abdul Sater (H)

Genitourinary Malignancies Branch, National Cancer Institute, Bethesda, MD, USA.

Brian J Capaldo (BJ)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA.

Ravi A Madan (RA)

Genitourinary Malignancies Branch, National Cancer Institute, Bethesda, MD, USA.

James L Gulley (JL)

Genitourinary Malignancies Branch, National Cancer Institute, Bethesda, MD, USA.

Guinevere Chun (G)

Genitourinary Malignancies Branch, National Cancer Institute, Bethesda, MD, USA.

Maria J Merino (MJ)

Laboratory of Pathology, National Cancer Institute, Bethesda, MD, USA.

Peter A Pinto (PA)

Urologic Oncology Branch, National Cancer Institute, Bethesda, MD, USA.

Daniela C Salles (DC)

Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Harsimar B Kaur (HB)

Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Tamara L Lotan (TL)

Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.

David J Venzon (DJ)

Biostatistics and Data Management Section, National Cancer Institute, Rockville, MD, USA.

Peter L Choyke (PL)

Molecular Imaging Branch, National Cancer Institute, Bethesda, MD, USA.

Baris Turkbey (B)

Molecular Imaging Branch, National Cancer Institute, Bethesda, MD, USA.

William L Dahut (WL)

Genitourinary Malignancies Branch, National Cancer Institute, Bethesda, MD, USA.

Adam G Sowalsky (AG)

Laboratory of Genitourinary Cancer Pathogenesis, National Cancer Institute, Bethesda, MD, USA. Electronic address: adam.sowalsky@nih.gov.

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