Choline transporter-like proteins 1 and 2 are newly identified plasma membrane and mitochondrial ethanolamine transporters.
Animals
Antigens, CD
/ chemistry
Biological Transport
Cell Line
Cell Membrane
/ metabolism
Ethanolamine
/ metabolism
Humans
Membrane Glycoproteins
/ chemistry
Membrane Transport Proteins
/ chemistry
Mitochondria
/ metabolism
Models, Molecular
Organic Cation Transport Proteins
/ chemistry
Protein Conformation
CTL1
CTL2
ethanolamine transport
membranes
phospholipids
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
Historique:
received:
26
08
2020
revised:
24
03
2021
accepted:
26
03
2021
pubmed:
1
4
2021
medline:
21
8
2021
entrez:
31
3
2021
Statut:
ppublish
Résumé
The membrane phospholipids phosphatidylcholine and phosphatidylethanolamine (PE) are synthesized de novo by the CDP-choline and CDP-ethanolamine (Kennedy) pathway, in which the extracellular substrates choline and ethanolamine are transported into the cell, phosphorylated, and coupled with diacylglycerol to form the final phospholipid product. Although multiple transport systems have been established for choline, ethanolamine transport is poorly characterized and there is no single protein assigned a transport function for ethanolamine. The solute carriers 44A (SLC44A) known as choline transporter-like proteins-1 and -2 (CTL1 and CTL2) are choline transporter at the plasma membrane and mitochondria. We report a novel function of CTL1 and CTL2 in ethanolamine transport. Using the lack or the gain of gene function in combination with specific antibodies and transport inhibitors we established two distinct ethanolamine transport systems of a high affinity, mediated by CTL1, and of a low affinity, mediated by CTL2. Both transporters are Na
Identifiants
pubmed: 33789160
pii: S0021-9258(21)00384-7
doi: 10.1016/j.jbc.2021.100604
pmc: PMC8081925
pii:
doi:
Substances chimiques
Antigens, CD
0
Membrane Glycoproteins
0
Membrane Transport Proteins
0
Organic Cation Transport Proteins
0
SLC44A1 protein, human
0
SLC44A2 protein, human
0
Ethanolamine
5KV86114PT
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
100604Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
Références
Nat Commun. 2020 Jul 13;11(1):3479
pubmed: 32661250
Mol Cell Biol. 2007 May;27(9):3327-36
pubmed: 17325045
Cell Metab. 2015 May 5;21(5):718-30
pubmed: 25955207
Mol Cancer Ther. 2020 Sep;19(9):1909-1921
pubmed: 32546662
J Biol Chem. 2018 Jul 13;293(28):10870-10883
pubmed: 29866881
Med Hypotheses. 2011 Dec;77(6):1083-7
pubmed: 21963355
Biochem Pharmacol. 2014 Apr 1;88(3):303-12
pubmed: 24530235
Biomol Ther (Seoul). 2018 Jul 1;26(4):399-408
pubmed: 29223141
J Biol Chem. 2013 Feb 8;288(6):4158-73
pubmed: 23250747
Thromb Res. 2018 Nov;171:155-159
pubmed: 30312801
Elife. 2020 Apr 21;9:
pubmed: 32314961
J Lipid Res. 1986 Nov;27(11):1205-13
pubmed: 3559386
Brain. 2019 Jan 1;143(1):94-111
pubmed: 31855247
Neurochem Res. 1986 Jan;11(1):29-36
pubmed: 3960270
Int J Mol Sci. 2013 Jan 25;14(2):2529-50
pubmed: 23354482
Can J Biochem Physiol. 1959 Aug;37(8):911-7
pubmed: 13671378
J Pharmacol Sci. 2016 Jun;131(2):101-9
pubmed: 27262903
J Biol Chem. 2014 Mar 28;289(13):9053-64
pubmed: 24519946
In Vitro Cell Dev Biol Anim. 2016 May;52(5):562-7
pubmed: 27083163
Front Nutr. 2018 Jul 10;5:61
pubmed: 30042948
Exp Biol Med (Maywood). 2019 May;244(8):655-662
pubmed: 30776907
J Thromb Haemost. 2020 Jul;18(7):1714-1727
pubmed: 32297475
Trends Endocrinol Metab. 2016 Aug;27(8):553-562
pubmed: 27370525
J Nutr Biochem. 2015 Nov;26(11):1221-34
pubmed: 26242921
J Thromb Haemost. 2020 Jul;18(7):1556-1558
pubmed: 32619343
J Lipid Res. 2007 Oct;48(10):2172-81
pubmed: 17646670
Biochem Cell Biol. 1992 Dec;70(12):1319-24
pubmed: 1299269
J Cell Physiol. 1988 Dec;137(3):571-6
pubmed: 3192633
J Lipid Res. 2008 Oct;49(10):2197-211
pubmed: 18583706
Lipids. 2014 Aug;49(8):731-44
pubmed: 24972900
Arch Biochem Biophys. 2009 Mar 1;483(1):90-8
pubmed: 19135976
Neurochem Int. 2016 Feb;93:40-50
pubmed: 26746385
Transfus Med Hemother. 2018 Oct;45(5):300-309
pubmed: 30498408
Biochim Biophys Acta Biomembr. 2017 Jun;1859(6):1156-1163
pubmed: 28336315
Exp Biol Med (Maywood). 2006 May;231(5):490-504
pubmed: 16636297
Oxid Med Cell Longev. 2017;2017:4829180
pubmed: 28785375
Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Jan;1862(1):102-113
pubmed: 27349299
Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Jan;1865(1):158434
pubmed: 30910690
Chem Phys Lipids. 2020 Aug;230:104914
pubmed: 32360136
J Cell Mol Med. 2019 Feb;23(2):702-710
pubmed: 30402908
FASEB J. 2015 May;29(5):1663-75
pubmed: 25466896
Genomics. 2016 Dec;108(5-6):201-208
pubmed: 27829169
FASEB J. 2009 Aug;23(8):2749-58
pubmed: 19357133
Biochim Biophys Acta. 1984 Jul 6;794(2):326-32
pubmed: 6733140
J Biol Chem. 2009 Oct 2;284(40):27077-89
pubmed: 19666474