Differential Methylation in the
Adolescent
Adult
Child
Child, Preschool
DNA Methylation
Death, Sudden, Cardiac
/ etiology
Female
Genetic Predisposition to Disease
/ etiology
Glutathione Transferase
/ genetics
Humans
Infant
Infant, Newborn
Male
Middle Aged
Regulatory Sequences, Nucleic Acid
/ genetics
Sudden Unexpected Death in Epilepsy
/ etiology
Exome Sequencing
/ methods
Whole Genome Sequencing
/ methods
Young Adult
DNA methylation
RNA
epigenetics
genetics
molecular autopsy
sudden cardiac death
sudden unexpected death in epilepsy
sudden unexplained death
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
10 Mar 2021
10 Mar 2021
Historique:
received:
30
01
2021
revised:
27
02
2021
accepted:
04
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
4
5
2021
Statut:
epublish
Résumé
Sudden cardiac death (SCD) is a diagnostic challenge in forensic medicine. In a relatively large proportion of the SCDs, the deaths remain unexplained after autopsy. This challenge is likely caused by unknown disease mechanisms. Changes in DNA methylation have been associated with several heart diseases, but the role of DNA methylation in SCD is unknown. In this study, we investigated DNA methylation in two SCD subtypes, sudden unexplained death (SUD) and sudden unexpected death in epilepsy (SUDEP). We assessed DNA methylation of more than 850,000 positions in cardiac tissue from nine SUD and 14 SUDEP cases using the Illumina Infinium MethylationEPIC BeadChip. In total, six differently methylated regions (DMRs) between the SUD and SUDEP cases were identified. The DMRs were located in proximity to or overlapping genes encoding proteins that are a part of the glutathione S-transferase (GST) superfamily. Whole genome sequencing (WGS) showed that the DNA methylation alterations were not caused by genetic changes, while whole transcriptome sequencing (WTS) showed that DNA methylation was associated with expression levels of the
Identifiants
pubmed: 33801838
pii: ijms22062790
doi: 10.3390/ijms22062790
pmc: PMC7999472
pii:
doi:
Substances chimiques
glutathione S-transferase T1
EC 2.5.1.-
Glutathione Transferase
EC 2.5.1.18
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Novo Nordic Foundation
ID : NFF18OC0031634
Organisme : Allen and Aage Andersen's Foundation
ID : X
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