Two repeated motifs enriched within some enhancers and origins of replication are bound by SETMAR isoforms in human colon cells.
Coevolution
DNA binding
DNA replication
Domestication
Expression enhancer
MITE
Transposon
Journal
Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
04
08
2020
revised:
25
03
2021
accepted:
30
03
2021
pubmed:
5
4
2021
medline:
1
4
2022
entrez:
4
4
2021
Statut:
ppublish
Résumé
Setmar is a gene specific to simian genomes. The function(s) of its isoforms are poorly understood and their existence in healthy tissues remains to be validated. Here we profiled SETMAR expression and its genome-wide binding landscape in colon tissue. We found isoforms V3 and V6 in healthy and tumour colon tissues as well as incell lines. In two colorectal cell lines SETMAR binds to several thousand Hsmar1 and MADE1 terminal ends, transposons mostly located in non-genic regions of active chromatin including in enhancers. It also binds to a 12-bp motifs similar to an inner motif in Hsmar1 and MADE1 terminal ends. This motif is interspersed throughout the genome and is enriched in GC-rich regions as well as in CpG islands that contain constitutive replication origins. It is also found in enhancers other than those associated with Hsmar1 and MADE1. The role of SETMAR in the expression of genes, DNA replication and in DNA repair are discussed.
Identifiants
pubmed: 33812898
pii: S0888-7543(21)00124-5
doi: 10.1016/j.ygeno.2021.03.032
pii:
doi:
Substances chimiques
Protein Isoforms
0
Histone-Lysine N-Methyltransferase
EC 2.1.1.43
SETMAR protein, human
EC 2.1.1.43
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1589-1604Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.