Limonene-carvacrol: A combination of monoterpenes with enhanced antileishmanial activity.


Journal

Toxicology in vitro : an international journal published in association with BIBRA
ISSN: 1879-3177
Titre abrégé: Toxicol In Vitro
Pays: England
ID NLM: 8712158

Informations de publication

Date de publication:
Aug 2021
Historique:
received: 20 11 2020
revised: 16 03 2021
accepted: 02 04 2021
pubmed: 7 4 2021
medline: 19 11 2021
entrez: 6 4 2021
Statut: ppublish

Résumé

Leishmaniasis is a parasitosis with a wide incidence in developing countries. The drugs which are indicated for the treatment of this infection usually are able to promote high toxicity. A combination of limonene and carvacrol, monoterpenes present in plants with antiparasitic activity may constitute an alternative for the treatment of these diseases. In this study, the antileishmania activity against Leishmania major, cytotoxicity tests, assessment of synergism, parasite membrane damage tests as well as molecular docking and immunomodulatory activity of limonene-carvacrol (Lim-Car) combination were evaluated. The Lim-Car combination (5:0; 1:1; 1:4; 2:3; 3:2; 4:1 and 0:5) showed potential antileishmania activity, with mean inhibitory concentration (IC The 4:1 Lim-Car combination appears to be a promising candidate as a monotherapeutic antileishmania agent.

Sections du résumé

BACKGROUND BACKGROUND
Leishmaniasis is a parasitosis with a wide incidence in developing countries. The drugs which are indicated for the treatment of this infection usually are able to promote high toxicity.
PURPOSE OBJECTIVE
A combination of limonene and carvacrol, monoterpenes present in plants with antiparasitic activity may constitute an alternative for the treatment of these diseases.
METHODS METHODS
In this study, the antileishmania activity against Leishmania major, cytotoxicity tests, assessment of synergism, parasite membrane damage tests as well as molecular docking and immunomodulatory activity of limonene-carvacrol (Lim-Car) combination were evaluated.
RESULTS RESULTS
The Lim-Car combination (5:0; 1:1; 1:4; 2:3; 3:2; 4:1 and 0:5) showed potential antileishmania activity, with mean inhibitory concentration (IC
CONCLUSION CONCLUSIONS
The 4:1 Lim-Car combination appears to be a promising candidate as a monotherapeutic antileishmania agent.

Identifiants

pubmed: 33823240
pii: S0887-2333(21)00083-7
doi: 10.1016/j.tiv.2021.105158
pii:
doi:

Substances chimiques

Antiprotozoal Agents 0
Cymenes 0
Drug Combinations 0
Immunologic Factors 0
Protozoan Proteins 0
carvacrol 9B1J4V995Q
Limonene 9MC3I34447
NADH, NADPH Oxidoreductases EC 1.6.-
trypanothione reductase EC 1.8.1.12
DNA-Directed DNA Polymerase EC 2.7.7.7

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

105158

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Rita de Cássia Viana de Carvalho (RCV)

Laboratory of Antileishmania Activity, Medicinal Plants Research Center, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina 64049-550, Piauí, Brazil.

Valéria Carlos de Sousa (VC)

Laboratory of Antileishmania Activity, Medicinal Plants Research Center, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina 64049-550, Piauí, Brazil.

Laíz Pinheiro Santos (LP)

Laboratory of Antileishmania Activity, Medicinal Plants Research Center, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina 64049-550, Piauí, Brazil.

Ingredy Lopes Dos Santos (ILD)

Laboratory of Antileishmania Activity, Medicinal Plants Research Center, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina 64049-550, Piauí, Brazil.

Roseane Costa Diniz (RC)

Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, Maranhão, Brazil.

Raiza Raianne Luz Rodrigues (RRL)

Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, Maranhão, Brazil.

Maria das Graças Freire de Medeiros (MDGF)

Department of Pharmacy, Federal University of Piaui, Teresina 64049-550, Piauí, Brazil.

Klinger Antonio da Franca Rodrigues (KADF)

Laboratory of Infectious Diseases, Campus Ministro Reis Velloso, Federal University of Delta do Parnaíba, 64202-020 Parnaíba, PI, Brazil.

Michel Muálem de Moraes Alves (MMM)

Laboratory of Antileishmania Activity, Medicinal Plants Research Center, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina 64049-550, Piauí, Brazil; Department of Veterinary Morphophysiology, Federal University of Piauí, Teresina 64049-550, Piauí, Brazil.

Daniel Dias Rufino Arcanjo (DDR)

Laboratory of Antileishmania Activity, Medicinal Plants Research Center, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina 64049-550, Piauí, Brazil; Department of Biophysics and Physiology, Federal University of Piaui, 64049-550 Teresina, PI, Brazil. Electronic address: daniel.arcanjo@ufpi.edu.br.

Fernando Aécio de Amorim Carvalho (FAA)

Laboratory of Antileishmania Activity, Medicinal Plants Research Center, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina 64049-550, Piauí, Brazil; Department of Biochemistry and Pharmacology, Federal University of Piaui, 64049-550 Teresina, PI, Brazil.

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Classifications MeSH