A High Activity Level Is Required for Augmented Muscle Capillarization in Older Women.
Aged
Blood Platelets
/ chemistry
Body Composition
Capillaries
Cell Proliferation
Cross-Sectional Studies
Endothelial Cells
/ cytology
Exercise
/ physiology
Female
Femoral Artery
/ physiology
Humans
Leg
/ blood supply
Middle Aged
Mitochondria, Muscle
/ chemistry
Muscle, Skeletal
/ blood supply
Oxidative Phosphorylation
Oxygen Consumption
Postmenopause
Quadriceps Muscle
/ blood supply
Regional Blood Flow
Sedentary Behavior
Self Report
/ classification
Surveys and Questionnaires
Time Factors
Vascular Endothelial Growth Factor A
/ analysis
Journal
Medicine and science in sports and exercise
ISSN: 1530-0315
Titre abrégé: Med Sci Sports Exerc
Pays: United States
ID NLM: 8005433
Informations de publication
Date de publication:
01 05 2021
01 05 2021
Historique:
entrez:
12
4
2021
pubmed:
13
4
2021
medline:
3
7
2021
Statut:
ppublish
Résumé
This study aimed to evaluate the influence of lifelong regular physical activity on skeletal muscle capillarization in women. Postmenopausal women, 61±4 yr old, were divided according to self-reported physical activity level over the past 20 yrs: sedentary (SED; n = 14), moderately active (MOD; n = 12), and very active (VERY; n = 15). Leg blood flow (LBF) was determined by ultrasound Doppler, and blood samples were drawn from the femoral artery and vein for calculation of leg oxygen uptake (LVO2) at rest and during one-legged knee extensor exercise. A skeletal muscle biopsy was obtained from the vastus lateralis and analyzed for capillarization and vascular endothelial growth factor (VEGF) and mitochondrial OXPHOS proteins. Platelets were isolated from venous blood and analyzed for VEGF content and effect on endothelial cell proliferation. The exercise-induced rise in LBF and LVO2 was faster (P = 0.008) in VERY compared with SED and MOD. Steady-state LBF and LVO2 were lower (P < 0.04) in MOD and VERY compared with SED. Capillary-fiber ratio and capillary density were greater (P < 0.03) in VERY (1.65 ± 0.48 and 409.3 ± 57.5) compared with MOD (1.30 ± 0.19 and 365.0 ± 40.2) and SED (1.30 ± 0.30 and 356.2 ± 66.3). Skeletal muscle VEGF and OXPHOS complexes I, II, and V were ~1.6-fold and ~1.25-fold (P < 0.01) higher, respectively, in VERY compared with SED. Platelets from all groups induced an approximately nine-fold (P < 0.001) increase in endothelial cell proliferation. A very active lifestyle is associated with superior skeletal muscle exercise hemodynamics and greater potential for oxygen extraction concurrent with a higher skeletal muscle capillarization and mitochondrial capacity.
Identifiants
pubmed: 33844669
doi: 10.1249/MSS.0000000000002566
pii: 00005768-202105000-00003
doi:
Substances chimiques
Vascular Endothelial Growth Factor A
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
894-903Informations de copyright
Copyright © 2021 by the American College of Sports Medicine.
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