Two-photon fluorescent probe for cellular peroxynitrite: Fluorescence detection, imaging, and identification of peroxynitrite-specific products.

Fluorescent Dyes Hydrogen Peroxide Hypochlorous Acid Oxidation-Reduction Peroxynitrous Acid
Fluorescence imaging Fluorometric high throughput analyses Mass spectrometry Naphthalene-based probe Peroxynitrite Two-photon fluorescent probe

Journal

Free radical biology & medicine
ISSN: 1873-4596
Titre abrégé: Free Radic Biol Med
Pays: United States
ID NLM: 8709159

Informations de publication

Date de publication:
06 2021
Historique:
received: 04 02 2021
revised: 18 03 2021
accepted: 08 04 2021
pubmed: 17 4 2021
medline: 29 6 2021
entrez: 16 4 2021
Statut: ppublish

Résumé

A new naphthalene-based boronate probe, NAB-BE, for the fluorescence-based detection of inflammatory oxidants, including peroxynitrite, hypochlorous acid, and hydrogen peroxide, is reported. The chemical reactivity and fluorescence properties of the probe and the products are described. The major, phenolic oxidation product, NAB-OH, is formed in case of all three oxidants tested. This product shows green fluorescence, with a maximum at 512 nm, and can be excited either at 340 nm or in the near infrared region (745 nm) for two-photon fluorescence imaging. Peroxynitrite is the fastest of the oxidants tested and, in addition to the phenolic product, leads to the formation of a nitrated product, NAB-NO

Identifiants

DOI: 10.1016/j.freeradbiomed.2021.04.011 PMID: 33862158
pubmed: 33862158
pii: S0891-5849(21)00215-X
doi: 10.1016/j.freeradbiomed.2021.04.011
pii:
doi:

Substances chimiques

Fluorescent Dyes 0
Peroxynitrous Acid 14691-52-2
Hypochlorous Acid 712K4CDC10
Hydrogen Peroxide BBX060AN9V

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

24-35

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Aleksandra Grzelakowska (A)

Institute of Polymer and Dye Technology, Faculty of Chemistry, Lodz University of Technology, Stefanowskiego 12/16, 90-924, Lodz, Poland. Electronic address: aleksandra.grzelakowska@p.lodz.pl.

Monika Zielonka (M)

Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, United States. Electronic address: mzielonka@mcw.edu.

Karolina Dębowska (K)

Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland. Electronic address: karolina.debowska@p.lodz.pl.

Julia Modrzejewska (J)

Institute of Polymer and Dye Technology, Faculty of Chemistry, Lodz University of Technology, Stefanowskiego 12/16, 90-924, Lodz, Poland. Electronic address: julia.modrzejewska@dokt.p.lodz.pl.

Marcin Szala (M)

Institute of Polymer and Dye Technology, Faculty of Chemistry, Lodz University of Technology, Stefanowskiego 12/16, 90-924, Lodz, Poland. Electronic address: marcin.szala@p.lodz.pl.

Adam Sikora (A)

Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland. Electronic address: adam.sikora@p.lodz.pl.

Jacek Zielonka (J)

Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, United States. Electronic address: jzielonk@mcw.edu.

Radosław Podsiadły (R)

Institute of Polymer and Dye Technology, Faculty of Chemistry, Lodz University of Technology, Stefanowskiego 12/16, 90-924, Lodz, Poland. Electronic address: radoslaw.podsiadly@p.lodz.pl.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Two-photon fluorescent probe for cellular...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › Two-photon fluorescent probe for cellular...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Acide hypochloreux › Two-photon fluorescent probe for cellular...
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › Two-photon fluorescent probe for cellular...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'azote › Acide peroxynitreux › Two-photon fluorescent probe for cellular...