Glucan Conversion and Membrane Recovery of Biomimetic Cellulosomes During Lignocellulosic Biomass Hydrolysis.
Biomimetic cellulosomes
Enzyme immobilization
Lignocellulose hydrolysis
Membrane recovery
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
14
10
2020
accepted:
08
04
2021
pubmed:
20
4
2021
medline:
30
9
2021
entrez:
19
4
2021
Statut:
ppublish
Résumé
Enzyme immobilization has been identified as one way to recycle enzymes and reduce processing costs during enzymatic hydrolysis of lignocellulosic materials. However, most immobilization methods have not been attractive to lignocellulosic processing plants. In this study, cellulase enzymes were attached to a copolymer of glycidyl methacrylate (GMA) and poly(ethylene glycol) methyl ether methacrylate (PEGMA) to make polymer-enzyme conjugates (PECs) and facilitate recovery using a 50-kDa molecular weight cutoff membrane. Glucan conversion during biomass hydrolysis was investigated using new PECs and PECs recovered after an initial hydrolysis stage. Enzyme immobilization on PECs did not reduce effectiveness during the initial hydrolysis. Temperature and pH showed similar effects on free enzymes and PECs. PECs facilitated higher conversion rates than free enzymes at high biomass loadings. Recovered PECs were used to achieve approximately 100% glucan conversion in a subsequent hydrolysis step when supplemented with 40% of the free enzyme used in the first stage. The combination of PECs and membrane recovery has the potential to reduce hydrolysis cost during cellulosic bioprocessing.
Identifiants
pubmed: 33871766
doi: 10.1007/s12010-021-03569-x
pii: 10.1007/s12010-021-03569-x
doi:
Substances chimiques
Enzymes, Immobilized
0
Membranes, Artificial
0
lignocellulose
11132-73-3
Lignin
9005-53-2
Cellulase
EC 3.2.1.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2830-2842Subventions
Organisme : National Science Foundation
ID : CBET 1604422
Informations de copyright
© 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.
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