Screening of probiotic candidates in a simulated piglet small intestine in vitro model.
Animals
Bacteria
/ classification
Diarrhea
/ drug therapy
Enterotoxigenic Escherichia coli
/ pathogenicity
Escherichia coli Infections
/ drug therapy
Fatty Acids, Volatile
/ metabolism
Gastrointestinal Microbiome
/ drug effects
Intestine, Small
/ drug effects
Models, Biological
Probiotics
/ administration & dosage
Swine
in vitro model
Enterotoxigenic Escherichia coli
gut microbiota
piglet
probiotic, short-chain fatty acid
Journal
FEMS microbiology letters
ISSN: 1574-6968
Titre abrégé: FEMS Microbiol Lett
Pays: England
ID NLM: 7705721
Informations de publication
Date de publication:
01 05 2021
01 05 2021
Historique:
received:
21
08
2020
accepted:
16
04
2021
pubmed:
21
4
2021
medline:
3
11
2021
entrez:
20
4
2021
Statut:
ppublish
Résumé
The CoMiniGut in vitro model mimicking the small intestine of piglets was used to evaluate four probiotic strains for their potential as a preventive measure against development of diarrhea in weaned pigs. In the in vitro system, piglet digesta was inoculated with pathogenic enterotoxigenic Escherichia coli F4 (ETEC F4), and the short-chain fatty acid profile and the gut microbiota composition were assessed. A total of four probiotic strains were evaluated: Enterococcus faecium (CHCC 10669), Lactobacillus rhamnosus (CHCC 11994), Bifidobacterium breve (CHCC 15268) and Faecalibacterium prausnitzii (CHCC 28556). The significant differences observed in metabolite concetration and bacterial enumeration were attributed to variation in inoculating material or pathogen challenge rather than probiotic treatment. Probiotic administration influenced the microbiota composition to a small extend. Learnings from the present study indicate that the experimental setup, including incubation time and choice of inoculating material, should be chosen with care.
Identifiants
pubmed: 33877306
pii: 6240155
doi: 10.1093/femsle/fnab045
pii:
doi:
Substances chimiques
Fatty Acids, Volatile
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.