Biophysical Studies of the Binding of Viral RNA with the 80S Ribosome Using switchSENSE.
Biophysical Phenomena
Biosensing Techniques
/ methods
Dicistroviridae
/ physiology
Internal Ribosome Entry Sites
Kinetics
Models, Molecular
Proof of Concept Study
Protein Biosynthesis
RNA, Viral
/ chemistry
Ribosome Subunits, Large, Eukaryotic
/ chemistry
Ribosome Subunits, Small, Eukaryotic
/ chemistry
Saccharomyces cerevisiae
/ metabolism
Saccharomyces cerevisiae Proteins
/ chemistry
Biophysics
Kinetics
RNA
Ribosome
switchSENSE
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
20
4
2021
pubmed:
21
4
2021
medline:
23
6
2021
Statut:
ppublish
Résumé
Translation initiation, in both eukaryotes and bacteria, requires essential elements such as mRNA, ribosome , initiator tRNA, and a set of initiation factors. For each domain of life, canonical mechanisms and signals are observed to initiate protein synthesis. However, other initiation mechanism can be used, especially in viral mRNAs. Some viruses hijack cellular machinery to translate some of their mRNAs through a noncanonical initiation pathway using internal ribosome entry site (IRES), a highly structured RNAs which can directly recruit the ribosome with a restricted set of initiation factors, and in some cases even without cap and initiator tRNA. In this chapter, we describe the use of biosensors relying on electro-switchable nanolevers using the switchSENSE
Identifiants
pubmed: 33877606
doi: 10.1007/978-1-0716-1197-5_15
doi:
Substances chimiques
Internal Ribosome Entry Sites
0
RNA, Viral
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
341-350Références
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