Synthesis of a precursor of D-fagomine by immobilized fructose-6-phosphate aldolase.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
18
01
2021
accepted:
07
04
2021
entrez:
22
4
2021
pubmed:
23
4
2021
medline:
1
10
2021
Statut:
epublish
Résumé
Fructose-6-phosphate aldolase (FSA) is an important enzyme for the C-C bond-forming reactions in organic synthesis. The present work is focused on the synthesis of a precursor of D-fagomine catalyzed by a mutant FSA. The biocatalyst has been immobilized onto several supports: magnetic nanoparticle clusters (mNC), cobalt-chelated agarose (Co-IDA), amino-functionalized agarose (MANA-agarose) and glyoxal-agarose, obtaining a 29.0%, 93.8%, 89.7% and 53.9% of retained activity, respectively. Glyoxal-agarose FSA derivative stood up as the best option for the synthesis of the precursor of D-fagomine due to the high reaction rate, conversion, yield and operational stability achieved. FSA immobilized in glyoxal-agarose could be reused up to 6 reaction cycles reaching a 4-fold improvement in biocatalyst yield compared to the non-immobilized enzyme.
Identifiants
pubmed: 33886681
doi: 10.1371/journal.pone.0250513
pii: PONE-D-21-01852
pmc: PMC8062046
doi:
Substances chimiques
Enzymes, Immobilized
0
Fructosephosphates
0
Imino Pyranoses
0
Magnetite Nanoparticles
0
Cobalt
3G0H8C9362
fagomine
53185-12-9
fructose-6-phosphate
6814-87-5
Sepharose
9012-36-6
Aldehyde-Lyases
EC 4.1.2.-
fructose-6-phosphate phosphoketolase
EC 4.1.2.22
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0250513Déclaration de conflit d'intérêts
The authors have read the journal’s policy and have the following competing interests: SK and DM are co-founders of Nanos SCI and are listed on the Nanos SCI website. There are no patents, products in development or marketed products associated with this research to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
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