Coordinating the morphogenesis-differentiation balance by tweaking the cytokinin-gibberellin equilibrium.


Journal

PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074

Informations de publication

Date de publication:
04 2021
Historique:
received: 30 12 2020
accepted: 06 04 2021
revised: 06 05 2021
pubmed: 27 4 2021
medline: 28 8 2021
entrez: 26 4 2021
Statut: epublish

Résumé

Morphogenesis and differentiation are important stages in organ development and shape determination. However, how they are balanced and tuned during development is not fully understood. In the compound leaved tomato, an extended morphogenesis phase allows for the initiation of leaflets, resulting in the compound form. Maintaining a prolonged morphogenetic phase in early stages of compound-leaf development in tomato is dependent on delayed activity of several factors that promote differentiation, including the CIN-TCP transcription factor (TF) LA, the MYB TF CLAU and the plant hormone Gibberellin (GA), as well as on the morphogenesis-promoting activity of the plant hormone cytokinin (CK). Here, we investigated the genetic regulation of the morphogenesis-differentiation balance by studying the relationship between LA, CLAU, TKN2, CK and GA. Our genetic and molecular examination suggest that LA is expressed earlier and more broadly than CLAU and determines the developmental context of CLAU activity. Genetic interaction analysis indicates that LA and CLAU likely promote differentiation in parallel genetic pathways. These pathways converge downstream on tuning the balance between CK and GA. Comprehensive transcriptomic analyses support the genetic data and provide insights into the broader molecular basis of differentiation and morphogenesis processes in plants.

Identifiants

pubmed: 33901177
doi: 10.1371/journal.pgen.1009537
pii: PGENETICS-D-20-01955
pmc: PMC8102002
doi:

Substances chimiques

Cytokinins 0
Gibberellins 0
Plant Proteins 0
Transcription Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1009537

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Alon Israeli (A)

The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, Rehovot, Israel.

Yogev Burko (Y)

The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, Rehovot, Israel.

Sharona Shleizer-Burko (S)

The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, Rehovot, Israel.

Iris Daphne Zelnik (ID)

The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, Rehovot, Israel.

Noa Sela (N)

Department of Plant Pathology and Weed Research, Plant Protection Institute, Agricultural Research Organization, Volcani Institute, Rishon LeZion, Israel.

Mohammad R Hajirezaei (MR)

Molecular Plant Nutrition, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research, Seeland, Germany.

Alisdair R Fernie (AR)

Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.

Takayuki Tohge (T)

Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.

Naomi Ori (N)

The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, Rehovot, Israel.

Maya Bar (M)

The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University, Rehovot, Israel.
Department of Plant Pathology and Weed Research, Plant Protection Institute, Agricultural Research Organization, Volcani Institute, Rishon LeZion, Israel.

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