The anti-sigma factor MucA is required for viability in Pseudomonas aeruginosa.
AlgU
cystic fibrosis
envelope stress
sigma factor competition
Journal
Molecular microbiology
ISSN: 1365-2958
Titre abrégé: Mol Microbiol
Pays: England
ID NLM: 8712028
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
revised:
21
04
2021
received:
13
11
2020
accepted:
21
04
2021
pubmed:
28
4
2021
medline:
29
12
2021
entrez:
27
4
2021
Statut:
ppublish
Résumé
During decades-long infections in the cystic fibrosis (CF) airway, Pseudomonas aeruginosa undergoes selection. One bacterial genetic adaptation often observed in CF isolates is mucA mutations. MucA inhibits the sigma factor AlgU. Mutations in mucA lead to AlgU misregulation, resulting in a mucoid phenotype that is associated with poor CF disease outcomes. Due to its ability to be mutated, mucA is assumed to be dispensable for bacterial viability. Here we show that, paradoxically, a portion of mucA is essential in P. aeruginosa. We demonstrate that mucA is no longer required in a strain lacking algU, that mucA alleles encoding for proteins that do not bind to AlgU are insufficient for viability, and that mucA is no longer essential in mutant strains containing AlgU variants with reduced sigma factor activity. Furthermore, we found that overexpression of algU prevents cell growth in the absence of MucA, and that this phenotype can be rescued by the overproduction of RpoD, the housekeeping sigma factor. Together, these results suggest that in the absence of MucA, the inability to regulate AlgU activity results in the loss of bacterial viability. Finally, we speculate that the essentiality of anti-sigma factors that regulate envelope function may be a widespread phenomenon in bacteria.
Identifiants
pubmed: 33905139
doi: 10.1111/mmi.14732
pmc: PMC10069406
mid: NIHMS1880720
doi:
Substances chimiques
AlgU protein, Pseudomonas aeruginosa
0
Bacterial Proteins
0
MucA protein, Pseudomonas
0
Sigma Factor
0
rpoDA protein, Pseudomonas aeruginosa
137701-09-8
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
550-563Subventions
Organisme : NIAID NIH HHS
ID : K22 AI127473
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM114450
Pays : United States
Informations de copyright
© 2021 John Wiley & Sons Ltd.
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