The interaction between P2X3 and TRPV1 in the dorsal root ganglia of adult rats with different pathological pains.
Animals
Behavior, Animal
/ physiology
Capsaicin
/ analogs & derivatives
Ganglia, Spinal
/ drug effects
Inflammation
/ metabolism
Male
Neuralgia
/ metabolism
Neurons
/ drug effects
Pain
/ metabolism
Pain Measurement
Rats
Rats, Sprague-Dawley
Receptors, Purinergic P2X3
/ metabolism
Sensory System Agents
/ pharmacology
TRPV Cation Channels
/ metabolism
Transient receptor potential vanilloid 1
complete Freund's adjuvant
correlation effect
purinergic receptor P2X ligand-gated ion channel 3
spared nerve injury
Journal
Molecular pain
ISSN: 1744-8069
Titre abrégé: Mol Pain
Pays: United States
ID NLM: 101242662
Informations de publication
Date de publication:
Historique:
entrez:
28
4
2021
pubmed:
29
4
2021
medline:
1
12
2021
Statut:
ppublish
Résumé
Peripheral inflammatory and neuropathic pain are closely related to the activation of purinergic receptor P2X ligand-gated ion channel 3 (P2X3) and transient receptor potential vanilloid 1 (TRPV1), but the interaction between P2X3 and TRPV1 in different types of pathological pain has rarely been reported. In this study, complete Freund's adjuvant (CFA)-induced inflammatory pain and spared nerve injury (SNI)-induced neuropathic pain models were established in adult rats. The interactions between P2X3 and TRPV1 in the dorsal root ganglion were observed by pharmacological, co-immunoprecipitation, immunofluorescence and whole-cell patch-clamp recording assays. TRPV1 was shown to promote the induction of spontaneous pain caused by P2X3 in the SNI model, but the induction of spontaneous pain behaviour by TRPV1 was not completely dependent on P2X3
Identifiants
pubmed: 33906494
doi: 10.1177/17448069211011315
pmc: PMC8108079
doi:
Substances chimiques
Receptors, Purinergic P2X3
0
Sensory System Agents
0
TRPV Cation Channels
0
Trpv1 protein, rat
0
capsazepine
LFW48MY844
Capsaicin
S07O44R1ZM
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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