SspABCD-SspFGH Constitutes a New Type of DNA Phosphorothioate-Based Bacterial Defense System.
DNA phosphorothioate modification
SspFGH defense
additive defense
phage resistance
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
27 04 2021
27 04 2021
Historique:
entrez:
28
4
2021
pubmed:
29
4
2021
medline:
8
10
2021
Statut:
epublish
Résumé
Unlike nucleobase modifications in canonical restriction-modification systems, DNA phosphorothioate (PT) epigenetic modification occurs in the DNA sugar-phosphate backbone when the nonbridging oxygen is replaced by sulfur in a double-stranded (ds) or single-stranded (ss) manner governed by DndABCDE or SspABCD, respectively. SspABCD coupled with SspE constitutes a defense barrier in which SspE depends on sequence-specific PT modifications to exert its antiphage activity. Here, we identified a new type of ssDNA PT-based SspABCD-SspFGH defense system capable of providing protection against phages through a mode of action different from that of SspABCD-SspE. We provide further evidence that SspFGH damages non-PT-modified DNA and exerts antiphage activity by suppressing phage DNA replication. Despite their different defense mechanisms, SspFGH and SspE are compatible and pair simultaneously with one SspABCD module, greatly enhancing the protection against phages. Together with the observation that the
Identifiants
pubmed: 33906925
pii: mBio.00613-21
doi: 10.1128/mBio.00613-21
pmc: PMC8092258
pii:
doi:
Substances chimiques
ATP Binding Cassette Transporter, Subfamily D
0
Bacterial Proteins
0
DNA, Bacterial
0
Phosphates
0
thiophosphoric acid
TYM4M7EWCW
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 Wang et al.
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