Initiation and amplification of SnRK2 activation in abscisic acid signaling.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 04 2021
28 04 2021
Historique:
received:
14
10
2020
accepted:
29
03
2021
entrez:
29
4
2021
pubmed:
30
4
2021
medline:
13
5
2021
Statut:
epublish
Résumé
The phytohormone abscisic acid (ABA) is crucial for plant responses to environmental challenges. The SNF1-regulated protein kinase 2s (SnRK2s) are key components in ABA-receptor coupled core signaling, and are rapidly phosphorylated and activated by ABA. Recent studies have suggested that Raf-like protein kinases (RAFs) participate in ABA-triggered SnRK2 activation. In vitro kinase assays also suggest the existence of autophosphorylation of SnRK2s. Thus, how SnRK2 kinases are quickly activated during ABA signaling still needs to be clarified. Here, we show that both B2 and B3 RAFs directly phosphorylate SnRK2.6 in the kinase activation loop. This transphosphorylation by RAFs is essential for SnRK2 activation. The activated SnRK2s then intermolecularly trans-phosphorylate other SnRK2s that are not yet activated to amplify the response. High-order Arabidopsis mutants lacking multiple B2 and B3 RAFs show ABA hyposensitivity. Our findings reveal a unique initiation and amplification mechanism of SnRK2 activation in ABA signaling in higher plants.
Identifiants
pubmed: 33911084
doi: 10.1038/s41467-021-22812-x
pii: 10.1038/s41467-021-22812-x
pmc: PMC8080645
doi:
Substances chimiques
Arabidopsis Proteins
0
SnRK2 protein, Arabidopsis
0
Abscisic Acid
72S9A8J5GW
Protein Kinases
EC 2.7.-
OST1 protein, Arabidopsis
EC 2.7.1.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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