Widespread Alternative Splicing Changes in Metastatic Breast Cancer Cells.
Alternative Splicing
/ genetics
Base Sequence
Breast Neoplasms
/ genetics
Cell Line, Tumor
Exons
/ genetics
Female
Gene Expression Regulation, Neoplastic
Humans
Nonsense Mediated mRNA Decay
/ genetics
Oncogene Proteins
/ genetics
Peptide Synthases
/ genetics
RNA Precursors
/ genetics
RNA, Messenger
/ genetics
Reproducibility of Results
Serine-Arginine Splicing Factors
/ genetics
Survival Analysis
DCUN1D5
alternative splicing
breast cancer
cancer metastasis
exon skipping
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
09 04 2021
09 04 2021
Historique:
received:
04
03
2021
revised:
05
04
2021
accepted:
06
04
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
21
10
2021
Statut:
epublish
Résumé
Aberrant alternative splicing (AS) is a hallmark of cancer and a potential target for novel anti-cancer therapeutics. Breast cancer-associated AS events are known to be linked to disease progression, metastasis, and survival of breast cancer patients. To identify altered AS programs occurring in metastatic breast cancer, we perform a global analysis of AS events by using RNA-mediated oligonucleotide annealing, selection, and ligation coupled with next-generation sequencing (RASL-seq). We demonstrate that, relative to low-metastatic, high-metastatic breast cancer cells show different AS choices in genes related to cancer progression. Supporting a global reshape of cancer-related splicing profiles in metastatic breast cancer we found an enrichment of RNA-binding motifs recognized by several splicing regulators, which have aberrant expression levels or activity during breast cancer progression, including SRSF1. Among SRSF1-regulated targets we found
Identifiants
pubmed: 33918758
pii: cells10040858
doi: 10.3390/cells10040858
pmc: PMC8070448
pii:
doi:
Substances chimiques
Oncogene Proteins
0
RNA Precursors
0
RNA, Messenger
0
SRSF1 protein, human
0
Serine-Arginine Splicing Factors
170974-22-8
DCUN1D5 protein, human
EC 6.3.2.-
Peptide Synthases
EC 6.3.2.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Education and the National Research Foundation of Korea
ID : 2017R1A2B2005896
Organisme : Ministry of Education and the National Research Foundation of Korea
ID : 2020R1A2C2004682
Organisme : inistry of Education and the National Research Foundation of Korea
ID : 2019R1I1A1A01057372
Organisme : GIST Research Institute (GRI) IIBR" grant funded by the GIST
ID : 2020
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : IG 2018 Id.21966
Organisme : AIRC fellowship
ID : Davide Pradella
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